Том 23, № 1-2 (2023)

The paper presents the results of using multiplicative indices for species identification of polyploid green toads of Central Asia. Live adult individuals of Bufotes baturae (24 females and 17 males) from the territory of Tajikistan and B. pewzowi (14 females and 24 males) from Kazakhstan, Uzbekistan and Tajikistan were studied. The animals were caught in nature, measured and immediately released. The species belonging of the studied green toads was established by cytogenetic methods. According to the results of discriminant analysis, six proportionality indices were significant for the species identification of females. The females of B. batura and B. pewzowi formed separate non-overlapping clusters in the discriminant function space, and the level of their reliable classification was 100%. For the identification of B. batura and B. pewzowi males, four significant body proportionality indices were identified using discriminant analysis. The clusters of these two species in the space of discriminant function touched, and the level of correct classification was 97.6%. As a result of our research, one multiplicative index common to males and females was identified for highly reliable identification of B. baturae and B. pewzowi, as well as one multiplicative index separately for males and females of the studied species. The authors conclude that multiplicative indices based on standard morphometric indicators can be used for the practical determination of polyploid green toads. 

The results of our comparative analysis of parameters of the leukocyte blood system of underyearlings and mature specimens of the common viper Vipera berus are presented. The material was the blood of 36 females and 26 males caught in the Middle Volga basin, and 13 underyearlings born in captivity from 4 females of this sample. V. b. berus underyearlings differed: from the females by a lower content of heterophils and azurophils, an increased content of eosinophils and basophils, an increase in the leukocyte shift index, a decrease in the lymphocytic–granulocytic index, the index of the of heterophil–eosinophil ratio, the index of the lymphocyte–eosinophil ratio; from the males by a reduced content of heterophils, an increased content of eosinophils and basophils, an increase in the leukocyte shift index, the index of the heterophil–eosinophil ratio, and the index of the lymphocyte–eosinophil ratio. In general, the adaptive response of the blood system of underyearlings indicated the later activation of lymphocytes as an effector link and the imbalance of the immune response due to the lack of a resource of immunocompetent cells (agranulocytes) compared with the indices of adults from natural populations.

The paper presents the results of laboratory reproduction of Regel’s racerunner (Eremias regeli). Offspring was obtained from natural individuals (2 females and 2 males) captured in the vicinity of the Bokhtar town (Khatlon region, Republic of Tajikistan). Captive-born animals (3 females and 2 males) began laying eggs at the age of 7 months. The lizards from nature reproduced twice a year, while the animals obtained in the laboratory lay eggs up to three times per season. The clutches of both natural and laboratory lizards contained from 2 to 4 eggs. In total, 9 fertile clutches and 26 eggs were studied, which 18 newborn lizards were obtained from. The mass of one clutch ranged from 28.97 to 54.68% of the female mass. The eggs had a length of 12.3–18.1 mm, a width of 7.3–9.4 mm and a weight of 0.53–0.95 g. Incubation lasted 56–99 days. The newborn lizards had a body length of 26.5–35.1 mm, a tail length of 41.1–60.0 mm and a mass of 0.60–1.11 g. Comparing the reproductive parameters of E. regeli with other representatives of the tribus Eremiadini, the authors note relatively small sizes of adult females, their low fertility, but large sizes of eggs and newborn juveniles.