Отправить статью по E-mail HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera
- Авторы: Stepanova E.S.1, Makarenkova L.M.1, Chistyakov V.V.1, Rybakov Y.L.2, Gukasov V.M.2, Fedotcheva T.A.3,4, Parshin V.A.3, Votyakov V.A.3, Shimanovskii N.L.3
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Учреждения:
- People’s Friendship University of Russia (RUDN University)
- Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
- All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
- Выпуск: Том 52, № 12 (2019)
- Страницы: 1016-1020
- Раздел: Structure of Chemical Compounds, Methods of Analysis and Process Control
- URL: https://ogarev-online.ru/0091-150X/article/view/245584
- DOI: https://doi.org/10.1007/s11094-019-01944-x
- ID: 245584
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Аннотация
An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied.
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E. Stepanova
People’s Friendship University of Russia (RUDN University)
Автор, ответственный за переписку.
Email: stepanova25@inbox.ru
Россия, Moscow, 117198
L. Makarenkova
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Россия, Moscow, 117198
V. Chistyakov
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Россия, Moscow, 117198
Yu. Rybakov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Россия, Moscow, 123995
V. Gukasov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Россия, Moscow, 123995
T. Fedotcheva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF; All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
Email: stepanova25@inbox.ru
Россия, Moscow, 117997; Moscow, 113628
V. Parshin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Россия, Moscow, 117997
V. Votyakov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Россия, Moscow, 117997
N. Shimanovskii
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Россия, Moscow, 117997
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