Enviar artigo por via de e-mail HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera
- Autores: Stepanova E.S.1, Makarenkova L.M.1, Chistyakov V.V.1, Rybakov Y.L.2, Gukasov V.M.2, Fedotcheva T.A.3,4, Parshin V.A.3, Votyakov V.A.3, Shimanovskii N.L.3
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Afiliações:
- People’s Friendship University of Russia (RUDN University)
- Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
- All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
- Edição: Volume 52, Nº 12 (2019)
- Páginas: 1016-1020
- Seção: Structure of Chemical Compounds, Methods of Analysis and Process Control
- URL: https://ogarev-online.ru/0091-150X/article/view/245584
- DOI: https://doi.org/10.1007/s11094-019-01944-x
- ID: 245584
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Resumo
An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied.
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Sobre autores
E. Stepanova
People’s Friendship University of Russia (RUDN University)
Autor responsável pela correspondência
Email: stepanova25@inbox.ru
Rússia, Moscow, 117198
L. Makarenkova
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Rússia, Moscow, 117198
V. Chistyakov
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Rússia, Moscow, 117198
Yu. Rybakov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Rússia, Moscow, 123995
V. Gukasov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Rússia, Moscow, 123995
T. Fedotcheva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF; All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
Email: stepanova25@inbox.ru
Rússia, Moscow, 117997; Moscow, 113628
V. Parshin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Rússia, Moscow, 117997
V. Votyakov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Rússia, Moscow, 117997
N. Shimanovskii
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Rússia, Moscow, 117997
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