Email this article HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera
- Authors: Stepanova E.S.1, Makarenkova L.M.1, Chistyakov V.V.1, Rybakov Y.L.2, Gukasov V.M.2, Fedotcheva T.A.3,4, Parshin V.A.3, Votyakov V.A.3, Shimanovskii N.L.3
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Affiliations:
- People’s Friendship University of Russia (RUDN University)
- Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
- All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
- Issue: Vol 52, No 12 (2019)
- Pages: 1016-1020
- Section: Structure of Chemical Compounds, Methods of Analysis and Process Control
- URL: https://ogarev-online.ru/0091-150X/article/view/245584
- DOI: https://doi.org/10.1007/s11094-019-01944-x
- ID: 245584
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Abstract
An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied.
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About the authors
E. S. Stepanova
People’s Friendship University of Russia (RUDN University)
Author for correspondence.
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
L. M. Makarenkova
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
V. V. Chistyakov
People’s Friendship University of Russia (RUDN University)
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
Yu. L. Rybakov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 123995
V. M. Gukasov
Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 123995
T. A. Fedotcheva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF; All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117997; Moscow, 113628
V. A. Parshin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117997
V. A. Votyakov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117997
N. L. Shimanovskii
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117997
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