Email this article Peculiarities of placental microRNA expression in pregnancies complicated by gestational diabetes mellitus and preeclampsia
- Authors: Pakin V.S.1, Vashukova E.S.1, Kapustin R.V.1, Arzhanova O.N.1, Glotov A.S.1, Baranov V.S.1
-
Affiliations:
- FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
- Issue: Vol 66, No 3 (2017)
- Pages: 110-115
- Section: Articles
- URL: https://ogarev-online.ru/jowd/article/view/6844
- DOI: https://doi.org/10.17816/JOWD663110-115
- ID: 6844
Cite item
Abstract
The aim of study was to determine feasible changes of placental miRNAs expression profiles revealed by next generation sequencing (NGS) in pregnancies with GDM complicated or not with PE. Out of 27 miRNAs, studied expression was significantly different (FDR < 0.05) only for his-miR-45a. Comparative analysis of revealed reliable differences in expression of hsa-miR-4532 (p < 0.0001, FDR = 0.0008), hsa-miR-34c-5p (p < 0.0001, FDR = 0.0083), and hsa-miR-193b-5p (p < 0.0001, FDR = 0.0139) in pregnancy complicated by PE, without of GDM. The present results suggest that GDM and PE are associated with specific alterations in the placental miRNA expression profiles. Further studies are needed to verify the role of these microRNA in molecular mechanisms underlying GDM and PE pathogenesis.
Full Text
##article.viewOnOriginalSite##About the authors
Vladimir S. Pakin
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Author for correspondence.
Email: pakins@list.ru
PhD student
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034Elena S. Vashukova
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Email: vi_lena@list.ru
scientist
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034Roman V. Kapustin
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Email: kapustin.roman@gmail.com
PhD
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034Olga N. Arzhanova
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Email: arjanova_olga@mail.ru
head of the Department Pathology of pregnancy, professor
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034Andrey S. Glotov
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Email: anglotov@mail.ru
senior researcher of the Laboratory prenatal diagnosis of hereditary and congenital diseases
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034Vladislav S. Baranov
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Email: baranov@vb2475.spb.ed
head of the Laboratory prenatal diagnosis of hereditary and congenital diseases, Corr. RAS, Professor
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034References
- Петрухин В.А., Бурумкулова Ф.Ф. Гестационный сахарный диабет // Архив акушерства и гинекологии им. В.Ф. Снегирева. – 2014. – № 1. – С. 48–51. [Petrukhin VA, Burumkulova FF. Gestatsionnyi sakharnyi diabet. Arkhiv akusherstva i ginekologii im. VF Snegireva. 2014;1(1):48-51. (In Russ.)]
- American Diabetes Association. Classification and Diagnosis of Diabetes. Diabetes Care. 2016;39(Suppl1): S13-S22.
- Айламазян Э.К., Мозговая Е.В. Гестоз: теория и практика. – М.: А36 МЕДпресс-информ, 2008. [Aylamazyan EK, Mozgovaya EV. Gestoz: teoriya i praktika. Moscow: MEDpress inform; 2008. (In Russ.)]
- Zhao C, Dong J, Jiang T, et al. Early second-trimester serum miRNA profiling predicts gestational diabetes mellitus. Public Library of Science. 2011;6(8):23925. doi: 10.1371/journal.pone.0023925.
- Zhao Z, Moley KH, Gronowski AM. Diagnostic potential for miRNAs as biomarkers for pregnancy-specific diseases. Clin Biochem. 2013;46(10-11):953-60. doi: 10.1016/j.clinbiochem.2013.01.026.
- Chim SS, Shing TK, Hung EC, et al. Detection and characterization of placental microRNAs in maternal plasma. Clin Chem. 2008;54(3):482-490. doi: 10.1373/clinchem.2007.097972.
- Gu Y, Sun J, Groome LJ, Wang Y. Differential miRNA expression profiles between the first and third trimester human placentas. Am J Physiol Endocrinol Metab. 2013;304(8):836-43. doi: 10.1152/ajpendo.00660.2012.
- Дедов И.И., Краснопольский В.И., Сухих Г.Т. Российский национальный консенсус «Гестационный сахарный диабет: диагностика, лечение, послеродовое наблюдение» // Сахарный диабет. – 2012. – № 4. – С. 4–10. [Dedov II, Krasnopol’skii VI, Sukhikh GT. Rossiiskii natsional’nyi konsensus “Gestatsionnyi sakharnyi diabet: diagnostika, lechenie, poslerodovoe nablyudenie”. Sakharnyi diabet. 2012;(4):4-10. (In Russ.)]
- Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10(3):R25.1-R25.10. doi: 10.1186/gb-2009-10-3-r25.
- Benjamini Y, Drai D, Elmer G, et al. Controlling the false discovery rate in behavior genetics research. Behav Brain Res. 2001;125: 279-284.
- Li T, Mo X, Fu L, Xiao B, Guo J. Molecular mechanisms of long noncoding RNAs on gastric cancer. Oncotarget. 2016;7:8601-8612. doi: 10.18632/oncotarget.6926.
- Madsen H, Ditzel J. Blood-oxygen transport in first trimester of diabetic pregnancy. Acta Obstet Gynecol Scand. 1984;63:317-320.
- He P, Shao D, Ye M, Zhang G. Analysis of gene expression identifies candidate markers and pathways in pre-eclampsia. J Obstet Gynaecol. 2015;35(6):578-84. doi: 10.3109/01443615.2014.990430.
- Ikeda Y, Tanji E, Makino N. MicroRNAs associated with mitogen-activated protein kinase in human pancreatic cancer. Mol Cancer Res. 2012;10(2):259-69. doi: 10.1158/1541-7786.MCR-11-0035.
- Buchholz T, Lohse P, Rogenhofer N, et al. Polymorphisms in the ACE and PAI-1 genes are associated with recurrent spontaneous miscarriages. Hum Reprod. 2003;18(11):2473-2477.
- Lam KW, Chiu CN, Chung MK, et al. Glycodelin-A as a modulator of trophoblast invasion. Hum Reprod. 2009;24(9):2093-2103. doi: 10.1093/humrep/dep205.
- Savarimuthu Francis SM, Davidson MR, Tan ME, et al. MicroRNA-34c is associated with emphysema severity and modulates SERPINE1 expression. BMC Geno mics. 2014;15:88. doi: 10.1186/1471-2164-15-88.
Supplementary files
