Detection of the RNA for new multicomponent virus in patients with Crimean-Congo hemorrhagic fever in southern Russia
- Authors: Ternovoi V.A.1, Gladysheva A.V.1, Sementsova A.O.1, Zaykovskaya A.V.1, Volynkina A.S.2, Kotenev E.S.2, Agafonov A.P.1, Loktev V.B.1
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Affiliations:
- State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
- Stavropol Anti-plague Institute
- Issue: Vol 75, No 2 (2020)
- Pages: 129-134
- Section: INFECTIOUS DISEASES: CURRENT ISSUES
- URL: https://ogarev-online.ru/vramn/article/view/125732
- DOI: https://doi.org/10.15690/vramn1192
- ID: 125732
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Abstract
Background. Recently, a new multicomponent RNA-containing virus was described and called as Jingmen tick virus (JMTV) supposedly belonging to flaviviruses. A virus contains of four viral particles and JMTV was firstly isolated from ticks in China.
Aims. Detection viral RNA specific for JMTV complex, sequencing genome fragments and taxonomy identification novel virus from JMTV complex in patients with Crimean-Congo hemorrhagic fever (CCHF) from southern European part of Russia.
Methods. Panel of 20 randomly selected sera from patients with confirmed Crimean-Congo hemorrhagic fever was collected in 2016 and was used for detection JMTV and CCHF viral RNA by PCR with experimental primers. Subsequent sequencing of isolated fragments of viral genomes was used for identification JMTV and CCHF virus genetic materials and phylogenetic analyses.
Results. The RNA of the CCHF virus and JMTV were detected in sera of four patients. Sequencing of the PCR fragments from S segment CCHF virus were identifying these isolates as members of Europa 1 clade. The nucleotide sequences of segment 2 (GP glycoprotein) of novel JMTV isolates clustered together by phylogenetic analysis. The level nucleotide identity for discovered JMTV isolates was only about 81−82% with comparison to the previously described European variants (Kosovo) of JMTV.
Conclusions. The results allow us to conclude that in CCHF patients the RNA of the CCHF virus and RNA of the novel multicomponent JMTV flavivirus were detected in serum.
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##article.viewOnOriginalSite##About the authors
Vladimir A. Ternovoi
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Author for correspondence.
Email: tern@vector.nsc.ru
ORCID iD: 0000-0003-1275-171X
SPIN-code: 1328-5960
ResearcherId: A-6497-2014
Ph. D. in Biology
Russian Federation, Novosibirsk region, KoltsovoAnastasia V. Gladysheva
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Email: Nastya95.ru@list.ru
ORCID iD: 0000-0002-7396-3954
SPIN-code: 5214-3421
стажер-исследователь
Russian Federation, Novosibirsk region, KoltsovoAlexandra O. Sementsova
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Email: sementsova_ao@vector.nsc.ru
ORCID iD: 0000-0002-7188-5948
SPIN-code: 3387-9642
младший научный сотрудник
Russian Federation, Novosibirsk region, KoltsovoAnna V. Zaykovskaya
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Email: zaykovskaya_av@vector.nsc.ru
ORCID iD: 0000-0002-7188-5948
SPIN-code: 9339-6505
Ph. D. in Biology
Russian Federation, Novosibirsk region, KoltsovoAnna S. Volynkina
Stavropol Anti-plague Institute
Email: volyn444@mail.ru
ORCID iD: 0000-0001-5554-5882
SPIN-code: 3387-4340
Ph. D. in Biology
Russian Federation, 13-15, Sovetskaya street, Stavropol, 355035Egor S. Kotenev
Stavropol Anti-plague Institute
Email: egor_kotenev@mail.ru
ORCID iD: 0000-0002-8036-8926
SPIN-code: 1562-9580
Ph. D. in Biology
Russian Federation, 13-15, Sovetskaya street, Stavropol, 355035Alexander P. Agafonov
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Email: agafonov@vector.nsc.ru
ORCID iD: 0000-0003-2577-0434
SPIN-code: 2112-2920
Doct. of Biology
Russian Federation, Novosibirsk region, KoltsovoValeriy B. Loktev
State Research Center for Virology and Biotechnology “Vector” Rospotrebnadzor
Email: valeryloktev@gmail.com
ORCID iD: 0000-0002-0229-321X
SPIN-code: 3496-8857
Doct. of biology Professor
Russian Federation, Novosibirsk region, KoltsovoReferences
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