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Post a Comment Morphological basis for possibility of applying electrochemical method with use of nanotechnological biosensors in diagnosis of colorectal cancer
- Authors: Belkin A.N.1, Freind G.G.1, Kochetov A.G.2
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Affiliations:
- E.A. Vagner Perm State Medical University
- Institute of Laboratory Medicine
- Issue: Vol 38, No 3 (2021)
- Pages: 88-96
- Section: Methods of diagnosis and technologies
- URL: https://ogarev-online.ru/PMJ/article/view/76010
- DOI: https://doi.org/10.17816/pmj38388-96
- ID: 76010
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Abstract
Objective. To assess the efficacy of electrochemical method using biosensors for studying the activity of alkaline phosphatase as a marker of functional atypism in the fresh and formalin-fixed biopsies of the colorectal cancer by means of comparing with the results of morphological study.
Materials and methods. Electrochemical method was used to study the fragments of tumors (adenocarcinomas) of the colon and colon mucosa from 78 patients who underwent endoscopic study of the colon. The results of electrochemical and morphological studied were assessed and compared.
Results. In the material from 70 patients, a mean value of the current strength, obtained while studying the tumor fragments, was 49.2 (95 % CI 41.3 – 88.9) nA and 33.1 (95 % CI 9.5 – 44.2) nA in case of “fresh” and formalin-fixed biopsies, respectively. A mean value of the current strength, obtained while studying mucosa fragments, was 119.7 (95 % CI 96.8 – 167.1) nА and 59.6 (95 % CI 48.3 – 71) nA in case of “fresh” and formalin-fixed biopsies, respectively. While conducting immunohistochemical study, in the tumor tissue a low expression of alkaline phosphatase compared to unchanged mucosa was detected.
Conclusions. Electrochemical method applying nanotechnological biosensors was shown to be used for a rapid, direct estimation of alkaline phosphatase content in the biopsies of the colorectal carcinoma. The obtained results allow considering electrochemical method as an additional method for assessment of functional atypism of tumor cells.
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##article.viewOnOriginalSite##About the authors
A. N. Belkin
E.A. Vagner Perm State Medical University
Author for correspondence.
Email: belkinanton87@gmail.com
Lecturer, Department of Pathological Anatomy with Sectional Course
Russian Federation, PermG. G. Freind
E.A. Vagner Perm State Medical University
Email: belkinanton87@gmail.com
MD, PhD, Professor, Head of Department of Pathological Anatomy with Sectional Course
Russian Federation, PermA. G. Kochetov
Institute of Laboratory Medicine
Email: belkinanton87@gmail.com
MD, PhD, Professor, Rector
Russian Federation, MoscowReferences
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