Study of optimal method of Heterodera glycines sample preparation for molecular diagnostics

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In this study, it is optimized by the methods of preparing samples for the release of genetic material (DNA) from soybean cyst-forming nematode Heterodera glycines , which is necessary for accurate identification. Various modifications of ready-made reagent kits from Russian and foreign manufacturers were tested: DNA-Extrane-2, FitoSorb, TsytoSorb, ORB-GMO-A by Sintol LLC (Russia), Proba-GS, Proba-NK by AgroDiagnostika LLC, and QIAamp DNA Mini Kit by Qiagen (Germany). By testing the PCR method «In real time», the sensitivity of Heterodera glycines total DNA release was tested using a test system consisting of forward and reverse primers and a modified probe: SCNrtF (5’-AAATTCCAGGCCGCTATCTC-3’), SCNrtR (5’CGTGGACTGAACTGAACTGGACAAAG-3’), SCNrtP (5’-FAM/-TGGGCTGGGTGCTTCTAGAACTTTT-/ BHQ-1/-3’).

Sobre autores

Anton Ivanov

All-Russian Plant Quarantine Center

Autor responsável pela correspondência
Email: tonijons8@mail.ru
ORCID ID: 0009-0002-5361-6100
Código SPIN: 4537-6755

Junior researcher, Helminthology Laboratory, Testing Laboratory

32 Pogranichnaya st., Bykovo, 140150, Moscow region, Russian Federation

Galina Bondarenko

All-Russian Plant Quarantine Center

Email: reseachergm@mail.ru
ORCID ID: 0000-0002-1635-2508
Código SPIN: 2631-5209

Candidate of Biological Sciences, Senior researcher, Head of the Testing Laboratory Center

32 Pogranichnaya st., Bykovo, 140150, Moscow region, Russian Federation

Bibliografia

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