The role of Wnt and Shh signaling systems in noggin-induced tumorigenesis

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Abstract

The cross-interaction between BMP, Wnt and Shh signalling pathways in developing epithelial skin tumours remains poorly understood. To study the role of Wnt and Shh signalling pathways in tumour development upon BMP inhibition, we utilized a transgenic mouse model, overexpressing BMP antagonist noggin in the skin epithelium and leading to the development of hair follicle-derived tumours shortly after birth. Comparative gene and protein expression analyses revealed up-regulation of Wnt and Shh signalling systems in the skin of transgenic mice at various stages of follicular tumour development. Furthermore, recombinant BMP-4 suppresses the expression of Shh in the culture of tumor cells, while pharmacological inhibitors of Wnt and Shh significantly slow down the formation and development of tumors in noggin-expressing transgenic mice. These results enhance our knowledge about the role of growth factors in carcinogenesis and may lead to finding new targets for specific therapies for oncological diseases.

About the authors

Andrei N. Mardaryev

University of Bradford

Email: A.Mardaryev@bradford.ac.uk
ORCID iD: 0000-0002-7826-5506
SPIN-code: 2645-6478

PhD, Associate Professor, Centre for Sckin Sciences

Richmond Road, Bradford, West Yorkshire, BD7 1DP, United Kingdom

Nikita S. Mardaryev

Kazan State Medical University

Email: nikitamardarjev@yandex.ru
ORCID iD: 0009-0006-3723-2616
SPIN-code: 8791-1497

student

49 Butlerova st., Kazan, Republic of Tatarstan, 420012, Russian Federation

Nataliya V. Mardaryeva

Chuvash State Agrarian University

Author for correspondence.
Email: volga480@yandex.ru
ORCID iD: 0000-0001-7863-7245
SPIN-code: 5663-0732

Candidate of Biological Sciences, Associate Professor, Head of the Department of Biotechnology and Processing of Agricultural Products

29 Karla Marksa st., Cheboksary, Chuvash Republic, 428003, Russian Federation

Nadezda V. Schiptsova

Chuvash State Agrarian University

Email: shipnavars@mail.ru
ORCID iD: 0000-0002-7575-6297
SPIN-code: 4486-9698

Candidate of Biological Sciences, Associate Professor

29 Karla Marksa st., Cheboksary, Chuvash Republic, 428003, Russian Federation

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