Microsatellite analysis of Kalmyk cattle

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Abstract

Development of specialized beef cattle breeding contributes to increase in beef production, which directly affects the country’s food security. Currently, increasing productivity of animals is the major trend of cattle breeding development, which in turn requires improvement of breeding. The effectiveness of breeding work depends on the assessment of genetic value of breeding animals. To control authenticity of animal origin is a prerequisite for conducting breeding work. One of the main directions of cattle breeding in Kalmykia is breeding of Kalmyk cattle. The aim of the research was to study genetic diversity of Kalmyk cattle populations using microsatellite analysis. The study was conducted in the Regional Research and Production Center for Reproduction of Kalmyk State University. 60 Kalmyk cattle from ‘Plodovitoe’ agricultural production company in Maloderbetovsky district were studied. PCR analysis was performed by 9 microsatellite loci: BM1824, BM 2113, INRA023, SPS 115, TGLA 122, TGLA 126, TGLA 227, ETH 10, ETH 225. It was found that the average number of alleles was 10.1, while the number of alleles per locus varied from 7 (BM 1824, SPS 115, ETH 10) to 18 (TGLA 122). The loci with the largest range of alleles were BM 2113 (12), INRA 023 (12), TGLA 122 (18) and TGLA 227 (12). The most informative loci were INRA 023, TGLA 122 and TGLA 227. The level of observed heterozygosity varied from 0.67 (ETH 10) to 0.83 (SPS 115, TGLA 227, ETH 225), and expected heterozygosity - from 0.86 (BM 1824, SPS 115, ETH 10) to 0.92 (BM 2113, INRA 023, TGLA 227). Analysis of fixation index data showed that 8 loci had negative index (BM 1824 (-0.22), BM 2113 (-0.26), INRA 023 (-0.26), SPS 115 (-0.18), TGLA 122 (-0.12), TGLA 126 (-0.10), ETH 10 (-0.28), ETH 225 (-0.04) and 1 locus (TGLA 227) had positive index (1.0). The results of the analysis of microsatellite loci showed that level of genetic diversity in the studied herd of Kalmyk cattle is high.

About the authors

Viktoria S. Ubushieva

Kalmyk State University named after B.B. Gorodovikov

Author for correspondence.
Email: vicki_93g@mail.ru
ORCID iD: 0000-0003-0320-7771
SPIN-code: 1991-8614

Researcher, Youth laboratory

Elista, Republic of Kalmykia, 358000, Russian Federation

Ivan F. Gorlov

Volga Region Research Institute of Meat and Milk Production and Processing

Email: niimmp@mail.ru
ORCID iD: 0000-0002-8683-8159
SPIN-code: 8249-9437

Doctor of Agricultural Sciences, Academician of the Russian Academy of Sciences, Scientific director

6 imeni Marshala Rokossovskogo st., Volgograd, 400131, Russian Federation

Nadezhda V. Chimidova

Kalmyk State University named after B.B. Gorodovikov

Email: nadezhdatchimidova@yandex.ru
ORCID iD: 0000-0003-3043-091X
SPIN-code: 3221-7432

Head of the Youth laboratory

Elista, Republic of Kalmykia, 358000, Russian Federation

Altana V. Ubushieva

Kalmyk State University named after B.B. Gorodovikov

Email: ameli-altanas@mail.ru
ORCID iD: 0000-0002-9916-7972
SPIN-code: 7219-0185

Researcher, Youth laboratory

Elista, Republic of Kalmykia, 358000, Russian Federation

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