Neutrophil CD10 and CD16 as markers of generalized infection development in newborns
- Authors: Obraztsov I.V.1, Obraztsova A.A.1, Voronina O.V.1, Chernikova E.A.1, Mishchenko A.Y.1, Gordukova M.A.1, Davydova N.V.1, Zhirkova J.V.1,2, Korsunskiy A.A.1,3
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Affiliations:
- Speransky Children’s City Clinical Hospital No. 9
- Pirogov Russian National Research Medical University
- Sechenov First Moscow State Medical University
- Issue: Vol 14, No 3 (2024)
- Pages: 369-380
- Section: Original Study Articles
- URL: https://ogarev-online.ru/2219-4061/article/view/268211
- DOI: https://doi.org/10.17816/psaic1822
- ID: 268211
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Abstract
BACKGROUND: Neonatal sepsis remains a critical concern. Thus, predictors of infection development and generalization should be determined.
AIM: This study aimed to determine novel neutrophil surface biomarkers for early prediction of the infections in newborns.
MATERIALS AND METHODS: This observational, single-center, prospective, selective, uncontrolled, unblinded experimental study included 261 newborns, with a mean postconceptual age of 38.7 (38.4–39.0) weeks and a mean gestation age of 38.0 (37.7–38.2) weeks. Blood samples were collected into vacutainers on hospitalization day 1. Patients were enrolled between April 2022 and December 2023. The primary endpoints were length of stay in the ICU and total length of hospitalization in patients with normal and decreased values of CD16 and CD10 neutrophils and HLA-DR monocytes. The expression of CD16 on CD62Lhigh neutrophils, total neutrophil CD10, and monocyte HLA-DR were evaluated by flow cytometry.
RESULTS: We assessed infants in the “control” (n = 96), “localized infection” (n = 95), and “generalized infection” (n = 70) subgroups. In all patients, a decrease in CD16 was associated with an increase in the median ICU stay from 4 to 8 days (p = 9.33 × 10–8) and total stay from 14 to 22 days (p = 1.58 × 10–7). A decrease in CD10 was associated with an increase in median ICU stay from 4 to 8 days (p = 3.01 × 10–6) and in the total stay from 14 to 19 days (p = 2.78 × 10–5). A decrease in monocytic HLA-DR was associated with a longer ICU and total hospital stay: 4 vs 8 days (p = 7.16 × 10–5) and 14 vs 21 days (p = 4.03 × 10–5), respectively. The median ICU stay in patients with a decrease in CD16 but normal CD10 was 4 days, whereas a decrease in both indicators was associated with prolonged hospital stay to 11 days (p = 2.13 × 10–5). The median hospital stay in patients with decreased CD16 but normal CD10 was 16 days, whereas the drop of both markers was related with increase of hospitalization stay to 23 days (p = 3.36 × 10–6). In the “localized infection” subgroup, low CD16 was associated with an increased median ICU stay from 4 to 6 days (p = 0.010) and the median hospital stay from 13 to 19 days (p = 4.14 × 10–4). In the “generalized infection” subgroup, decreased CD10 was related with prolongation of the median ICU stay from 7 to 11 days (p = 0.011) and the median total duration of hospitalization from 19 to 27 days (p = 0.037).
CONCLUSIONS: A decrease in CD10 and CD16 on the neutrophils at the start of clinical is an unfavorable prognostic factor of infectious and septic complications in newborns.
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##article.viewOnOriginalSite##About the authors
Igor V. Obraztsov
Speransky Children’s City Clinical Hospital No. 9
Author for correspondence.
Email: obraztsoviv@zdrav.mos.ru
ORCID iD: 0000-0002-6649-853X
SPIN-code: 6466-5680
MD, Cand. Sci. (Medicine)
Russian Federation, 29 Shmitovskii av., Moscow, 123317Anastasiia A. Obraztsova
Speransky Children’s City Clinical Hospital No. 9
Email: obraztsovaaa@zdrav.mos.ru
MD
Russian Federation, 29 Shmitovskii av., Moscow, 123317Oksana V. Voronina
Speransky Children’s City Clinical Hospital No. 9
Email: tkachukviktor601@gmail.com
ORCID iD: 0009-0001-5942-2430
MD
Russian Federation, 29 Shmitovskii av., Moscow, 123317Ekaterina A. Chernikova
Speransky Children’s City Clinical Hospital No. 9
Email: krasenkova.ea@gmail.com
ORCID iD: 0000-0001-9409-7832
SPIN-code: 6346-9181
Russian Federation, 29 Shmitovskii av., Moscow, 123317
Anastasiya Yu. Mishchenko
Speransky Children’s City Clinical Hospital No. 9
Email: nasta.tlt@mail.ru
ORCID iD: 0009-0003-1353-1441
SPIN-code: 4509-8053
MD
Russian Federation, 29 Shmitovskii av., Moscow, 123317Maria A. Gordukova
Speransky Children’s City Clinical Hospital No. 9
Email: gordukovama@zdrav.mos.ru
ORCID iD: 0000-0002-3948-8491
SPIN-code: 2205-4033
MD, Cand. Sci. (Medicine)
Russian Federation, 29 Shmitovskii av., Moscow, 123317Nataliia V. Davydova
Speransky Children’s City Clinical Hospital No. 9
Email: nata1902@yandex.ru
ORCID iD: 0000-0002-7325-6045
SPIN-code: 9997-6197
MD, Cand. Sci. (Medicine)
Russian Federation, 29 Shmitovskii av., Moscow, 123317Julia V. Zhirkova
Speransky Children’s City Clinical Hospital No. 9; Pirogov Russian National Research Medical University
Email: zhirkova@mail.ru
ORCID iD: 0000-0001-7861-6778
SPIN-code: 5560-6679
MD, Dr. Sci. (Medicine), Associate Professor
Russian Federation, 29 Shmitovskii av., Moscow, 123317; 117513, Moscow, st. Ostrovityanova, house 1Anatoly A. Korsunskiy
Speransky Children’s City Clinical Hospital No. 9; Sechenov First Moscow State Medical University
Email: dr_kaa@mail.ru
ORCID iD: 0000-0002-9087-1656
SPIN-code: 6374-0484
MD, Dr. Sci. (Medicine), Professor
Russian Federation, 29 Shmitovskii av., Moscow, 123317; 119991, Moscow, Bolshaya Pirogovskaya st., building 2, building 4References
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