Email this article Analysis of NAD and NAD-Dependent Protein Deacetylation in Mouse Tissues
- Authors: Solovjeva L.V.1, Panchenko A.V.2, Shabalin K.A.3, Nerinovski K.B.4, Yakimov A.P.3, Gubareva E.A.2, Svetlova M.P.1, Mudrak O.S.1, Khodorkovskiy M.A.5, Nikiforov A.A.1,5, Kulikova V.A.1,5
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Affiliations:
- Institute of Cytology, Russian Academy of Sciences
- Petrov National Medical Research Center of Oncology, Ministry of Health of the Russian Federation
- Konstantinov Petersburg Nuclear Physics Institute of the National Research Center Kurchatov Institute
- St. Petersburg State University
- Peter the Great St. Petersburg Polytechnic University
- Issue: Vol 12, No 6 (2018)
- Pages: 491-495
- Section: Article
- URL: https://ogarev-online.ru/1990-519X/article/view/212808
- DOI: https://doi.org/10.1134/S1990519X18060123
- ID: 212808
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Abstract
Nicotinamide adenine dinucleotide (NAD) plays a key role in the vital metabolic and regulatory processes in mammals. Disturbance of the NAD level regulation is associated with the development of such serious diseases as pellagra, neurodegenerative and cardiovascular disorders, diabetes, cancer and others. This paper presents an experimental approach that allows to determine the amount of NAD+ in mouse tissues using NMR spectroscopy, as well as the level of NAD+-dependent deacetylation of proteins in the cytosol and mitochondria.
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About the authors
L. V. Solovjeva
Institute of Cytology, Russian Academy of Sciences
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 194064
A. V. Panchenko
Petrov National Medical Research Center of Oncology, Ministry of Healthof the Russian Federation
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 197758
K. A. Shabalin
Konstantinov Petersburg Nuclear Physics Institute of the National Research CenterKurchatov Institute
Email: veronika.a.kulikova@gmail.com
Russian Federation, Gatchina, 188300
K. B. Nerinovski
St. Petersburg State University
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 199034
A. P. Yakimov
Konstantinov Petersburg Nuclear Physics Institute of the National Research CenterKurchatov Institute
Email: veronika.a.kulikova@gmail.com
Russian Federation, Gatchina, 188300
E. A. Gubareva
Petrov National Medical Research Center of Oncology, Ministry of Healthof the Russian Federation
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 197758
M. P. Svetlova
Institute of Cytology, Russian Academy of Sciences
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 194064
O. S. Mudrak
Institute of Cytology, Russian Academy of Sciences
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 194064
M. A. Khodorkovskiy
Peter the Great St. Petersburg Polytechnic University
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 195251
A. A. Nikiforov
Institute of Cytology, Russian Academy of Sciences; Peter the Great St. Petersburg Polytechnic University
Author for correspondence.
Email: andrey.nikiforov@gmail.com
Russian Federation, St. Petersburg, 194064; St. Petersburg, 195251
V. A. Kulikova
Institute of Cytology, Russian Academy of Sciences; Peter the Great St. Petersburg Polytechnic University
Author for correspondence.
Email: veronika.a.kulikova@gmail.com
Russian Federation, St. Petersburg, 194064; St. Petersburg, 195251
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