Selectin expression and severity of nitrosylating stress in patients with acute venous thrombosis of the lower extremities
- Autores: Korotkova N.V.1, Kalinin R.E.1, Suchkov I.A.1, Mzhavanadze N.D.1, Povarov V.O.1, Nikiforov A.A.1
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Afiliações:
- Ryazan State Medical University named after academician I.P. Pavlov
- Edição: Volume 28, Nº 3 (2025)
- Páginas: 29-37
- Seção: Biological chemistry
- URL: https://ogarev-online.ru/1560-9596/article/view/284917
- DOI: https://doi.org/10.29296/25877313-2025-03-04
- ID: 284917
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Resumo
Introduction. Acute venous thrombosis is a socially significant pathology, and therefore the molecular basis of its pathogenesis is attracting close attention from modern scientists. At the same time, the adhesive function performed by CD62P, CD62E and CD62L molecules has not been sufficiently studied, especially under conditions of changes in the synthesis of nitric oxide (II).
The aim. To evaluate the effect of nitric oxide on changes in the expression of intercellular adhesion molecules CD62P, CD62E and CD62L, based on markers of nitrosylating stress in the blood serum of patients with acute venous thrombosis.
Material and methods. Guided by the design of the study, two groups were formed: control – 30 clinically healthy donors and experimental – 30 patients with acute venous thrombosis of the lower extremities. The ELISA sandwich method was used for quantitative analysis of blood serum for CD62P, CD62E and CD62L and nitric oxide (II) synthases, the competitive ELISA method for the determination of nitrothyrosine (NT). Nitric oxide metabolites (nitrates and nitrites) were evaluated using the Metelskaya V.A. method. The formation of bityrosine crosslinking was recorded by the intensity of fluorescent glow.
Results. The concentration of CD62P in the blood serum of healthy donors and patients with acute venous thrombosis was 51.83 [35.13; 80.35] and 131.18 [110.70; 164.32], respectively (p=0.001), which was statistically significantly lower than in healthy individuals; CD62E and CD62L tended to decrease. The concentration of nitric oxide metabolites was 43.15[33.50; 51.12] and 122.8 [71.21; 164.79], respectively (p=0.001), other markers of nitrosylating stress showed multidirectional changes. A direct positive correlation of average strength was found between CD62L and the concentration of nitrates and nitrites.
Conclusion. Nitrosylating stress affects the adhesive function of the endothelium during the formation of a blood clot in the veins, which is accompanied by decreased expression of CD62P, CD62E and CD62L and a multidirectional change in the concentration of marker molecules HC – nitrothyrosine and bityrosine. At the same time, an increase in NOx is noted, which acts as an auxiliary pathway for the synthesis of NO (II). The noted transformations confirm the integration of nitric oxide metabolism and the expression of CD62L, CD62E and CD62L in hypercoagulation.
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N. Korotkova
Ryazan State Medical University named after academician I.P. Pavlov
Autor responsável pela correspondência
Email: fnv8@yandex.ru
ORCID ID: 0000-0001-7974-2450
Código SPIN: 3651-3813
Ph.D. (Med.), Associate Professor of the Department of Biological Chemistry, Senior Research Scientist at the Central Research Laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026R. Kalinin
Ryazan State Medical University named after academician I.P. Pavlov
Email: kalinin-re@yandex.ru
ORCID ID: 0000-0002-0817-9573
Código SPIN: 5009-2318
Dr.Sc. (Med.), Professor, Head of the Department of Cardiovascular, Endovascular Surgery, and Diagnostic Radiology
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026I. Suchkov
Ryazan State Medical University named after academician I.P. Pavlov
Email: i.suchkov@rzgmu.ru
ORCID ID: 0000-0002-1292-5452
Código SPIN: 6473-8662
Dr.Sc. (Med.), Professor, Professor of the Department of Cardiovascular, Endovascular Surgery, and Diagnostic Radiology
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026N. Mzhavanadze
Ryazan State Medical University named after academician I.P. Pavlov
Email: nina_mzhavanadze@mail.ru
ORCID ID: 0000-0001-5437-1112
Código SPIN: 7757-8854
Dr.Sc. (Med.), Associate Professor, Professor of the Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology, Senior Research Scientist at the Central Research Laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026V. Povarov
Ryazan State Medical University named after academician I.P. Pavlov
Email: povarov.vladislav@mail.ru
ORCID ID: 0000-0001-8810-9518
Código SPIN: 2873-1391
Ph.D. (Med.), Associate Professor, Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026A. Nikiforov
Ryazan State Medical University named after academician I.P. Pavlov
Email: alnik003@yandex.ru
ORCID ID: 0000-0001-9742-4528
Código SPIN: 8366-5282
Ph.D. (Med.), Associate Professor of the Department of Pharmacology, Head of the Central Research Laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Bibliografia
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