Email this article New fructan — the O-specific polysaccharide from Escherichia coli O92
- Authors: Senchenkova S.N.1, Xu G.2, Qi Y.2, Perepelov A.V.1, Shashkov A.S.1, Liu B.2, Knirel Y.A.2
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Affiliations:
- N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
- Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University
- Issue: Vol 66, No 7 (2017)
- Pages: 1304-1307
- Section: Full Articles
- URL: https://ogarev-online.ru/1066-5285/article/view/241013
- DOI: https://doi.org/10.1007/s11172-017-1889-2
- ID: 241013
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Abstract
The structure of the O-specific polysaccharide (O-antigen) from Escherichia coli O92 was established. The acid-labile polysaccharide was produced by O-deacylation of the lipopolysaccharide under mild alkaline conditions. The methylation analysis combined with 1D and 2D 1H and 13C NMR spectroscopy demonstrated that the O-specific polysaccharide chain of the lipopolysaccharide is a linear fructan composed of mono-О-acetylated disaccharide repeating units having the structure →1)-β-d-Fruf3Ac-(2→3)-β-d-Fruf-(2→. To the best of our knowledge, this fructan has been hitherto unknown in bacteria.
About the authors
S. N. Senchenkova
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
Russian Federation, 47 Leninsky prosp., Moscow, 119991
Guangnan Xu
Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University
Email: yknirel@gmail.com
China, 23 Hongda Street, Tianjin, 300457
Yanfen Qi
Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University
Email: yknirel@gmail.com
China, 23 Hongda Street, Tianjin, 300457
A. V. Perepelov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
Russian Federation, 47 Leninsky prosp., Moscow, 119991
A. S. Shashkov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: yknirel@gmail.com
Russian Federation, 47 Leninsky prosp., Moscow, 119991
Bin Liu
Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University
Email: yknirel@gmail.com
China, 23 Hongda Street, Tianjin, 300457
Yu. A. Knirel
Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University
Author for correspondence.
Email: yknirel@gmail.com
China, 23 Hongda Street, Tianjin, 300457
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