Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy

Abstract

A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using the fluorochrome cycanine Cy5 and digital fluorescence microscopy. A method was developed to activate the carboxylic groups on the PET surface and to allow covalent immobilization of amino group-containing molecular constructs.