Enviar artigo por via de e-mail Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy
- Autores: Miftakhov R.A.1, Lapa S.A.1, Shershov V.E.1, Zasedateleva O.A.1, Guseinov T.O.1, Spitsyn M.A.1, Kuznetsova V.E.1, Mamaev D.D.1, Lysov Y.P.1, Barsky V.E.1, Timofeev E.N.1, Zasedatelev A.S.1, Chudinov A.V.1
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Afiliações:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Edição: Volume 63, Nº 4 (2018)
- Páginas: 512-518
- Seção: Molecular Biophysics
- URL: https://ogarev-online.ru/0006-3509/article/view/152673
- DOI: https://doi.org/10.1134/S0006350918040127
- ID: 152673
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Resumo
A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using the fluorochrome cycanine Cy5 and digital fluorescence microscopy. A method was developed to activate the carboxylic groups on the PET surface and to allow covalent immobilization of amino group-containing molecular constructs.
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Sobre autores
R. Miftakhov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
S. Lapa
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
V. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
O. Zasedateleva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
T. Guseinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
M. Spitsyn
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
V. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
D. Mamaev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
Yu. Lysov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
V. Barsky
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
E. Timofeev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
A. Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Rússia, Moscow, 119991
A. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Autor responsável pela correspondência
Email: chud@eimb.ru
Rússia, Moscow, 119991
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