Characterization of an Acellular Dermal Matrix Obtained by Different Detergent-Enzymatic Methods


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Acellular dermal matrixes (ADM) for reconstructive surgery were made by treating skin samples from rats and humans with sodium dodecylsulfate (SDS), sodium deoxycholate (DOC), saponin, and DNase. A mixture of SDS and DOC removed cell residues effectively, though the ADM had cytotoxic properties. After treatment with 2% DOC, there was no cytotoxic effect and the resulting material contained 27-202 ng/mg dry weight DNA and from 0.07 to 2.6% fat, depending on the source of the samples.

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N. Sarbaeva

Samara State Medical University, Ministry of Health of the Russian Federation

编辑信件的主要联系方式.
Email: sarbaeva@gmail.com
俄罗斯联邦, Samara

M. Milyakova

Samara State Medical University, Ministry of Health of the Russian Federation

Email: sarbaeva@gmail.com
俄罗斯联邦, Samara

V. Rossinskaya

Samara State Medical University, Ministry of Health of the Russian Federation

Email: sarbaeva@gmail.com
俄罗斯联邦, Samara

O. Gribkova

Samara State Medical University, Ministry of Health of the Russian Federation

Email: sarbaeva@gmail.com
俄罗斯联邦, Samara

D. Dolgushkin

Samara State Medical University, Ministry of Health of the Russian Federation

Email: sarbaeva@gmail.com
俄罗斯联邦, Samara

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