Analysis of circulating miRNA levels in coronary heart disease patients with varying degrees of cardiovascular complications risk. correlations with the MSCT-CA data
- 作者: Rozhkov A.N.1, Shchekochikhin D.Y.1, Baulina N.М.2, Matveeva N.A.2, Favorova O.O.2, Akselrod A.S.1, Tebenkova E.S.1, Gognieva D.G.1, Kopylov P.Y.1
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隶属关系:
- I.M. Sechenov First Moscow State Medical University (Sechenov University)
- National Medical Research Center of Cardiology
- 期: 卷 75, 编号 4 (2020)
- 页面: 283-291
- 栏目: MOLECULAR MEDICINE AND GENETICS: CURRENT ISSUES
- URL: https://ogarev-online.ru/vramn/article/view/125690
- DOI: https://doi.org/10.15690/vramn1325
- ID: 125690
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Rationale. Cardiovascular diseases remain the leading cause of human death in the world. Studying the role of regulatory non-coding RNAs, which include short single-stranded miRNA molecules, allows a more detailed understanding of the pathological processes underlying the progression of atherosclerosis.
Objective — to compare the levels of circulating miRNAs in patients with coronary heart disease, confirmed by multislice computed tomography-coronarography (MSCT-CA), with risks of cardiovascular complications and clinical and demographic characteristics. To compare the profiles of circulating miRNAs in groups of patients with stable and unstable atherosclerotic plaques.
Methods. MicroRNA levels in the plasma of peripheral blood of patients with coronary heart disease were determined using the miScript miRNA PCR Array MIHS-105Z kit (Qiagen). The significance of differences in miRNA levels between the compared groups was determined using the Mann–Whitney U-test. The correlations of the levels of circulating miRNAs with clinical and demographic parameters were evaluated using the Spearman correlation coefficient. Risk assessment of cardiovascular complications in these patients was carried out using validated scales (ACC/AHA, Framinghm, SCORE, MESA). Atherosclerotic plaque stability was evaluated using MSCT-CA.
Results. The study showed a significant (p < 0.05) decrease in miR-16, miR-211, miR-195 miRNA levels in the plasma of patients with coronary heart disease, which correlated with an increase in cardiac vascular risk (CVR) according to ACC/AHA, Framingham and MESA. When comparing groups of patients with stable and unstable atherosclerotic plaques, the latter revealed an increase in the level of let-7b-5p circulating microRNA (p < 0.05).
Conclusion. Significant associations of the three studied microRNAs with the estimated risk of CVR were identified. It is important to find circulating let-7b-5p in a group of patients with unstable atherosclerotic plaques. Correlations were established between the levels of circulating microRNAs and clinical and demographic characteristics of patients. The study shows the involvement of some microRNAs in the regulation of atherosclerosis.
作者简介
Andrey Rozhkov
I.M. Sechenov First Moscow State Medical University (Sechenov University)
编辑信件的主要联系方式.
Email: arozhkov@outlook.com
ORCID iD: 0000-0002-2735-076X
MD
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992Dmitriy Shchekochikhin
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: agishm@list.ru
ORCID iD: 0000-0002-8209-2791
SPIN 代码: 3753-6915
MD, PhD, Associate Professor
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992Natalia Baulina
National Medical Research Center of Cardiology
Email: tati.90@mail.ru
ORCID iD: 0000-0001-8767-2958
SPIN 代码: 4704-0104
MD, PhD in Biology, Research Associate
俄罗斯联邦, 15a, 3rd Cherepkovskaya street, Moscow, 121552Natalia Matveeva
National Medical Research Center of Cardiology
Email: natalijamat@rambler.ru
ORCID iD: 0000-0002-4369-2882
SPIN 代码: 8760-4002
PhD in Biology, Research Associate
俄罗斯联邦, 15a, 3rd Cherepkovskaya street, Moscow, 121552Olga Favorova
National Medical Research Center of Cardiology
Email: olga_favorova@mail.ru
ORCID iD: 0000-0002-5271-6698
SPIN 代码: 8500-7456
PhD in Biology, Proffessor
俄罗斯联邦, 15a, 3rd Cherepkovskaya street, Moscow, 121552Anna Akselrod
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: 7402898@mail.ru
ORCID iD: 0000-0003-3417-794X
SPIN 代码: 4566-7759
д.м.н., профессор
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992Ekaterina Tebenkova
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: flor670@yandex.ru
ORCID iD: 0000-0003-4991-675X
MD, PhD, Associate Professor
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992Daria Gognieva
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: dashkagog@mail.ru
ORCID iD: 0000-0002-0451-2009
SPIN 代码: 4011-5405
MD, PhD Student
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992Philipp Kopylov
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: fjk@inbox.ru
ORCID iD: 0000-0001-5124-6383
SPIN 代码: 8287-6897
MD, PhD, Professor
俄罗斯联邦, 8-2, Trubetskaya street, Moscow, 119992参考
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