Cholesterol loading test for diagnosing atherosclerosis with normolipoproteinemia

Clinical observations suggest that coronary atherosclerosis may develop against the background of normal blood lipid content - normolipoproteinemia. As a diagnostic method of atherosclerosis with normolipoproteinemia the authors suggest using fat load and determination of the activity of hydrolytic liver enzymes using antipyrine test. They revealed in such patients reduced fat tolerance and decreased activity of liver hydroxylases. However, these tests are insufficiently selective, since fat load cannot reflect cholesterol metabolism abnormalities, and the antipyrine test cannot determine cholesterol hydroxylase (CH) activity, since the latter are highly specific for the substrate.