Neustaedter. Modification of the Frank-Goldberger method for the determination of estrogenic substances in the blood (Endocrinologia Los Angelos, V. 2 (. XL. 1936)

A syringe is taken from the cubital vein 40 cubic meters. cm of blood and place it in a sterile Petri dish, in which 30 to 40 g of dehydrated sodium sulfate are poured, mix with a glass rod until a thick mass is obtained. The dried mass is transferred into a mortar and turned into powder. If the mixture does not thicken, then add more sodium sulfate. The powder is transferred to an Erlenmeyer flask and poured over 100 cubic meters. cm of ether, is mixed in a scupper for 20 minutes. Then the flask is placed on a special shelf that gives it a 45° tilt. The top layer of liquid is decanted and centrifuged twice in a row. The centrifuged ether is evaporated to dryness. The lipoid precipitate is dissolved in 6 cubic meters. cm of gasoline, to which 0.6 cu. see olive oil. The gasoline is allowed to evaporate. The olive oil, into which the lipoid extract has passed, is sterilized in an autoclave at 15 pounds of pressure for 15 minutes. When placed in a dark bottle with a ground-in stopper, this extract can be stored for several days. It is administered in fractionated doses to a sexually mature castrated mouse. On the first day, three injections are given at four hour intervals. The next day, two, at the same intervals. The extract is injected into the mouse under the skin of the back. Starting the next day, vaginal swabs are prepared for 4 consecutive days, twice a day. The smears are stained with 1% thionine.