A new method for isolating pure cultures

Abstract

Peterfi and Wamoscher (Zeit. Für Hygiene, 1926, Bd. 106, H. 1), in order to obtain pure cultures, carried out the finest manipulations with bacteria under control in Dunkelfeld: using a special chamber from glass slides and a micropipette, any bacterial cell and, transferring it to another similar chamber with a liquid nutrient medium, the process of reproduction of the microbe (Einzellkulturen) was observed under a microscope. Using this method, the authors also succeeded in infecting experimental animals with only one pneumococcal cell in a number of cases.

Full Text

##article.viewOnOriginalSite##