The level of inhibin A in the blood serum of women suffering from chronic trichomoniasis as a predictor of infertility

Aim. To determine the relationship between the level of inhibin A and biochemical factors of inflammation with the development of infertility in women suffering from chronic trichomoniasis.

Methods. The object of the study was the blood serum of 167 women suffering from chronic trichomoniasis. The level of inhibin A was determined by enzyme immunoassay. As a control, the blood serum of 22 practically healthy fertile women was used who had no history of trichomoniasis. The lymphocyte phenotype was determined using luminescent microscopy with monoclonal antibodies staining. The level of immunoglobulins in serum was determined by the standard Mancini method of radial immunodiffusion. The synthesis of cytokines was determined by the method of studying the spontaneous synthesis of interleukins-1β, -8, -10 and interferon γ. The level of cytokines and lactoferrin was evaluated by enzyme immunoassay. The concentrations of α2-macroglobulin and C-reactive protein were determined by the nephelometric method. The fibrinogen concentration was determined by the coagulometric method.

Results. In the group of patients with chronic trichomoniasis, a significant variability in the serum concentration of inhibin A from 4.3 to 29.4 ng/L was found. In a group of patients who had a reduced content of inhibin A, in 63.2% of cases, the development of secondary infertility was subsequently diagnosed; in the group of patients who had a normal level of inhibin A, secondary infertility developed only in 12.9% of cases.

Conclusion. It was found that among all acute phase proteins, only levels of C-reactive protein and lactoferrin have prognostic value and statistically significantly differ in groups of women with subsequently developed infertility and uncomplicated infertility trichomoniasis.