On the use of restorative indicators for the detection of cancer of the female genital organs

In recent years, there have been reports in the press about the use of restorative indicators for the diagnosis of malignant lesions of various human organs. Kueren et al. (1963) proposed a cytochemical reaction using methylene-triphenyltetrazole chloride (MTTC) for these purposes. For the diagnosis of cervical cancer, this reaction was used by Sirba et al. (1966), B. I. Zheleznov and L. S. Yezhova (1968). The basis of this dehydrogenase detection test is tetrazolium salts, which serve as electron acceptors from the oxidized substrate. In this case, tetrazolium is restored and its transition from a colorless soluble form to a colored precipitate — formazane or diformazane. One of the main properties of tetrazolium salts that determine their suitability for histochemical purposes is their ability to recover. Nitrosinium tetrazolium (nitro-CT) is recovered much more easily than other tetrazolium. That's why we used it for our reactions.