The effect of orexin A application on the reaction of microglia cells body size stimulated by LPS injection

Cover Page

Cite item

Abstract

The purpose of this study is to determine the effect of orexin A to LPS-activated microglia by investigating the morphological change. The effect of orexin on the level of LPS-activated microglia activation during the transition to the M1 phenotype was determined, which can be studied by measuring the cell area of the body.

About the authors

A P Synchikova

National Institute for Physiological Sciences, Okazaki; Institute of Experimental Medicine, Saint Petersburg

H Horiuchi

National Institute for Physiological Sciences, Okazaki

J Nabekura

National Institute for Physiological Sciences, Okazaki

References

  1. Hu X, Li P., Guo Y, et al. Microglia/macrophage polarization dynamics reveal novel mechanism of injury expansion after focal cerebral ischemia. Stroke. 2012;43(11):3063-70.
  2. Willie JT, Chemelli RM, Sinton CM, Yanagisawa M. To eat or to sleep? Orexin in the regulation on feeding and wakefulness. Annu Rev Neurosci. 2001;24:429-458.
  3. Xiong X, White RE, Xu L, et al. Mitigation of murine focal cerebral ischemia by the hypocretin/orexin system is associated with reduced inflammation. Stroke. 2013;44(3):764-70.
  4. Duffy CM, Yuan C, Wisdorf LE, et al. Role of orexin A signaling in dietary palmitic acid-activated microglial cells. Neurosci Lett. 2015;606:140-4.
  5. Shu Q, Hu Z-L, Huang C, et al. Orexin-A Promotes Cell Migration in Cultured Rat Astrocytes via Ca2+-Dependent PKCα and ERK1/2 Signals. PLoS One. 2014 Apr 18;9(4). https://doi.org/10.1371/journal.pone.0095259.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2019 Synchikova A.P., Horiuchi H., Nabekura J.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).