Email this article ANTIMICROBIAL ACTIVITY OF LYSOZYME TOWARDS LISTERIA MONOCYTOGENES AT VARIOUS MEDIUM CONDITIONS
- Authors: Berlov MN1, Legkovoy SV2, Umnyakova ES1, Kokryakov VN1,2
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Affiliations:
- Institute of Experimental Medicine, Saint Petersburg
- Saint Petersburg State University, Saint Petersburg
- Issue: Vol 19, No 1S (2019)
- Pages: 161-162
- Section: Articles
- URL: https://ogarev-online.ru/MAJ/article/view/19378
- ID: 19378
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Abstract
The in vitro antimicrobial action of human leukocyte lysozyme from on gram-positive bacterium Listeria monocytogenes under various medium conditions was studied. It was shown that in a low ionic strength buffer (without NaCl), lower doses of lysozyme are required to reveal the microbicidal effect than in the case of 0.075 or 0.15 M NaCl. The bacterial growth phase does not significantly affect the antimicrobial activity of lysozyme. The results obtained are consistent with the two-stage mechanism of the antimicrobial action of lysozyme, which includes enzymatic and non-enzymatic action.
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##article.viewOnOriginalSite##About the authors
M N Berlov
Institute of Experimental Medicine, Saint Petersburg
S V Legkovoy
Saint Petersburg State University, Saint Petersburg
E S Umnyakova
Institute of Experimental Medicine, Saint Petersburg
V N Kokryakov
Institute of Experimental Medicine, Saint Petersburg; Saint Petersburg State University, Saint Petersburg
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