Email this article THE CORTICOSTATIC EFFECT OF HUMAN LACTOFERRIN DEPENDS ON THE AMINO ACID COMPOSITION OF THE N-TERMINUS OF THE MOLECULE
- Authors: Aleshina GM1
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Affiliations:
- Institute of Experimental Medicine, Saint Petersburg
- Issue: Vol 19, No 1S (2019)
- Pages: 159-160
- Section: Articles
- URL: https://ogarev-online.ru/MAJ/article/view/19377
- ID: 19377
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Abstract
The effect of various structural variants of human lactoferrin on stress-induced changes in the corticosterone level in the blood in rats was studied. A model of combined emotional and physical stress - swimming in cold water (1-4 °C) for 2 minutes. The level of corticosterone in plasma was determined by enzyme immunoassay. We have previously found that preventive intraperitoneal administration of native lactoferrin reduces the stress-induced increase in corticosterone concentration in the blood of rats 30 minutes after stress. This work shows that transgenic human lactoferrin, which lacks four arginine residues at the N-terminus, does not have this effect. The obtained results allow us to conclude about the key role of N-terminal amino acid residues in the implementation of the corticostatic activity of lactoferrin.
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