Email this article Рathomorphological features of corneal regeneration in experimentally induced dystrophy against the background of intrastromal administration of autologous mononuclear leukocytes
- Authors: Denisko MS1, Krivosheina OI1, Filippova EO1, Mustafina LR1
-
Affiliations:
- Siberian State Medical University
- Issue: Vol 18, No 3 (2018)
- Pages: 57-63
- Section: Articles
- URL: https://ogarev-online.ru/MAJ/article/view/10933
- DOI: https://doi.org/10.17816/MAJ18357-63
- ID: 10933
Cite item
Open Access
Access granted
Subscription Access
Abstract
The purpose of research is study the features of the flow of reparative regeneration of the cornea in an experiment in vivo with induced bullous keratopathy after the introduction of a suspension of autologous mononuclear leukocytes in the posterior third of the corneal stroma. Materials and methods. The experiment was performed on 27 chinchilla rabbits (27 eyes). At the first stage of the experiment all animals were reproduced the model of corneal bullous keratopathy by mechanical damage and removal of its endothelial layer. At the second stage, 2 weeks after the development of the disease, the animals were divided, depending on the planned treatment, into 2 groups: the main, 12 animals once in the back third of the corneal stroma were administered a suspension of autologous mononuclear leukocytes; comparisons, 12 animals were treated by conservative therapy. Results. Reproduction of in vivo bullous keratopathy of the cornea by mechanical damage and removal of its endothelial layer is accompanied by rapid (within 2 weeks) the development of the disease with diffuse hydropic degeneration of the anterior epithelium, marked edema of the stroma with the bundle of collagen fibers. On the experimental model of the bullous keratopathy of cornea it was found that the introduction of a suspension of autologous mononuclear leukocytes in the posterior third of the corneal stroma in the treatment of the disease provides rapid (1.5 times) dehydration of the stroma, reduces the number of dystrophic cells in the anterior epithelium by 1.2 times compared with conservative treatment. The period of restoration of the normal thickness of the corneal slice in animals of the main group is reduced by 1.4 times. The conclusion. The introduction of autologous mononuclear leukocytes suspension into the posterior third of the corneal stroma in the treatment of experimental bullous keratopathy of cornea induces reparative regeneration, accelerating the restoration of damaged corneal structures and preventing its neovascularization.
Full Text
##article.viewOnOriginalSite##About the authors
M S Denisko
Siberian State Medical University
O I Krivosheina
Siberian State Medical University
E O Filippova
Siberian State Medical University
L R Mustafina
Siberian State Medical University
References
- Бикбов М.М., Шевчук Н.Е., Мальханов В.Б. Цитокины в клинической офтальмологии. - Уфа: Уфимский полиграфкомбинат, 2008. [Bikbov MM, Shevchuk NE, Mal’khanov VB. Tsitokiny v klinicheskoy oftal’mologii. Ufa: Ufimskiy poligrafkombinat; 2008. (In Russ.)]
- Запускалов И.В., Кривошеина О.И. Современная фармакотерапия язвенных поражений роговицы: применение аутологичных мононуклеаров крови. - Саабрюкен: Lambert Academic Publishing, 2013. [Zapuskalov IV, Krivosheina OI. Sovremennaya farmakoterapiya yazvennykh porazheniy rogovitsy: primenenie autologichnykh mononuklearov krovi. Saarbrücken: Lambert Academic Publishing; 2013. (In Russ.)]
- Каспарова Е.А., Суббот А.М., Антохин А.И., Павлюк А.С. Клиническая эффективность персонализированной клеточной терапии заболеваний эндотелия роговицы // Катарактальная и рефракционная хирургия. - 2011. - Т. 11. - № 2. - С. 45-49. [Kasparova EA, Subbot AM, Antokhin AI, Pavlyuk AS. Clinical efficacy of personalized cell therapy for endothelial keratitis. Kataraktal’naya i refraktsionnaya khirurgiya. 2011;11(2):45-49. (In Russ.)]
- Кетлинский С.А., Симбирцев А.С. Цитокины. - СПб.: Фолиант, 2008. [Ketlinskiy SA, Simbirtsev AS. Tsitokiny. Saint Petersburg: Foliant; 2008. (In Russ.)]
- Никитин Н.А., Кузбеков Ш.Р. Роль TGFβ в офтальмологии // Цитокины и воспаление. - 2009. - Т. 8. - № 1. - С. 3-9. [Nikitin NA, Kuzbekov SR. The role of TGFβ in ophthalmology. Cytokines & inflammation. 2009;8(1):3-9. (In Russ.)]
- Каспарова Е.А., Суббот А.М. Пролиферативный потенциал заднего эпителия роговицы человека // Вестник офтальмологии. - 2013. - Т. 129. - № 3. - С. 82-88. [Kasparova EA, Subbot AM. Proliferative potential of human corneal endothelium. Annals of ophtalmology. 2013;129(3):82-88. (In Russ.)]
- Слепова О.С. Патогенетическая роль цитокинов при различных заболеваниях глаз как основа для прогнозирования и выбора тактики иммунокорригирующего лечения // Российский офтальмологический журнал. - 2008. - Т. 1. - № 3. - С. 36-42. [Slepova OS. The pathogenetic role of cytokines in various eye diseases as a basis for prognostication and the choice of immune correction tactics. Rossiiskii oftal’mologicheskii zhurnal. 2008;1(3):36-42. (In Russ.)]
- Jeon HS, Yi K, Chung TY, et al. Chemically injured keratocytes induce cytokine release by human peripheral mononuclear cells. Cytokine. 2012;59(2):280-285. doi: 10.1016/j.cyto.2012.04.006.
Supplementary files
