Comparative Study of the Antimicrobial Activity of Lactic Acid Bacteria Strains against Pseudomonas aeruginosa

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Introduction: Pseudomonas aeruginosa, capable of causing food spoilage, has multifactorial resistance to various antimicrobial agents and disinfectants, which leads to food safety problems. In this regard, it is necessary to search for and develop new effective methods to control P. aeruginosa contamination in food processing enterprises. Preparations based on lactic acid bacteria synthesizing various antimicrobial compounds can be considered as an alternative to chemical disinfectants and preservatives.Purpose is a comparative evaluation of antimicrobial activity of different strains of lactic acid bacteria in relation to representatives of P. aeruginosa isolated from different sources.Materials and methods: the objects of the study were strains of lactic acid bacteria (LAB) Lactobacillus helveticus, Streptococcus thermophilus, Lactococcus lactis and Lacticaseibacillus paracasei from the collection of FGANU VNIMI. The antimicrobial activity of the strains under study was evaluated against the type test strain P. aeruginosa ATCC 25668. Antimicrobial activity was investigated by co-culture method in accordance with MU 2.3.2.2789-10. For the lactic acid bacteria strains that showed the highest antimicrobial activity against P. aeruginosa ATCC 25668, the antimicrobial activity against wild strains of P. aeruginosa 42, P. aeruginosa М1 and P. aeruginosa 47 was additionally investigated.Results: Differences in the degree of antagonistic activity of lactic acid bacteria representatives in relation to the collection strain of P. aeruginosa and wild-type isolates were shown. The high efficiency of lactobacilli representatives belonging to the species L. helveticus, in particular strain L. helveticus Bbn4, as an antimicrobial agent against strains of P. aeruginosa of both collection and wild type was confirmed.Conclusion: The results of the study showed that representatives of lactobacilli, in particular L. helveticus, had high inhibitory activity against strains of collection and wild type P. aeruginosa, isolated from different sources, and can be considered as promising antimicrobial agents against such a complex pathogen as P. aeruginosa. In particular, the strain with the highest inhibitory activity, L. helveticus Bbn4, may be a potential antagonist strain with a broad spectrum of antimicrobial activity. However, further studies are needed to identify the mechanisms of antimicrobial action of this culture.

About the authors

Svetlana Anatolyevna Kishilova

All-Russian Dairy Research Institute

Email: s_kishilova@vnimi.org
ORCID iD: 0009-0000-9498-4757

Viktoria Alexandrovna Leonova

All-Russian Dairy Research Institute

Email: v_leonova@vnimi.org
ORCID iD: 0000-0003-2691-8859

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