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Search and verification of Betula nana L. hybrid samples in the Urals
- Authors: Medvedeva S.O.1, Cherepanova O.E.1, Petrova I.V.1, Teptina A.Y.2
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Affiliations:
- Botanical Garden, UrB RAS, Ekaterinburg, Russian Federation
- Ural Federal University, Ekaterinburg, Russian Federation
- Issue: Vol 521, No 1 (2025)
- Pages: 193-197
- Section: Articles
- URL: https://ogarev-online.ru/2686-7389/article/view/306233
- DOI: https://doi.org/10.31857/S2686738925020047
- ID: 306233
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Abstract
Using the flow cytometry method, hybrid samples were verified in B. nana populations, most of which were identified in the northern part of the Ural Mountains. Analysis of the ITS1 region nucleotide sequences obtained using the Sanger method showed low differentiation of B. nana and B. pubescens species associated with current and ancestral hybridization processes. Analysis of intragenomic polymorphism of ITS1 sequences using Illumina technology revealed the presence of rDNA of both B. nana and B. pubescens in first-generation interspecific hybrids genome.
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About the authors
S. O. Medvedeva
Botanical Garden, UrB RAS, Ekaterinburg, Russian Federation
Email: so.medvedeva@gmail.com
O. E. Cherepanova
Botanical Garden, UrB RAS, Ekaterinburg, Russian Federation
I. V. Petrova
Botanical Garden, UrB RAS, Ekaterinburg, Russian Federation
A. Y. Teptina
Ural Federal University, Ekaterinburg, Russian Federation
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