Methods of modeling portal vein catheterization in small laboratory animals and subsequent intraportal correction of liver function in patients with obstructive jaundice

the improvement of methods for modeling portal vein catheterization and the further development of intraportal methods for correcting liver function are promising areas of scientific research that can make a significant contribution to understanding the pathogenesis and treatment of liver diseases. The experiment was conducted on 35 sexually mature male Wistar rats, divided into 2 groups: the control group (n=10), the experimental group (n=25). In the control and experimental groups, the animals underwent surgical intervention to create obstructive jaundice and portal vein catheterization. Against the background of mechanical jaundice, the animals were intraportally injected with a solution of sodium heparin, and the animals of the experimental group, in addition to heparin, were injected with an ethoxidol solution for 8 days. For dynamic monitoring of the condition of animals with obstructive jaundice, venous blood was collected on the 3rd, 9th and 11th days of follow-up after choledochal decompression. In order to assess the functional activity of the liver, the concentrations of total bilirubin (OB), alanine and aspartic aminotransferases (AlAT, AsAT), and alkaline phosphatase (ALP) were studied. Combination therapy with sodium heparin and ethoxidol has shown positive results in the form of reduced damage to liver cells, decreased activity of the enzymes ALP and AlAT. Analysis of liver structural changes on the 11th day of follow-up after the use of sodium heparin in combination with an antioxidant showed a decrease in liver morphological disorders compared with the control group in the form of a decrease in hepatocyte edema, as well as its structural zone.

mechanical jaundice, portal vein catheterization, intraportal administration of drugs, laboratory animals, ethoxidol, antioxidant agents