Clinical significance of immunohistochemical expression of «early» HPV proteins in the diagnosis of squamous intraepithelial lesions of the cervix and uterus of mild degree

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Abstract

This study evaluated the immunohistochemical expression of early HPV proteins (E1, E2, E4) for the differential diagnosis of cervical SIL. A cross-sectional study was conducted from 2022–2023 on 128 HPV-positive patients aged 18–50 years, divided into 4 groups based on histological diagnosis (no lesions, chronic cervicitis, LSIL, HSIL). Analysis showed that a history of LSIL for 2 or more years was statistically significantly more frequent in patients with HSIL (46.7%). Expression of E2 protein in the upper 2/3 layers of the cervical epithelium was more often observed in LSIL (87.5%), and in the upper 1/3 of the cervical epithelium, in HSIL (66.7%). Expression of E1+E4 proteins was statistically significantly more frequent in LSIL (50%). The sensitivity and specificity of IHC expression of HPV E2 proteins in the upper 2/3 layers of the squamous epithelium of the cervix and E1+E4 for LSIL were 87.5% and 92.9%, and 50% and 91%, respectively. The obtained results indicate the importance of using early HPV proteins E1, E2, E4 for the differential diagnosis of LSIL.

About the authors

D. A. Dobrovolskaya

FSBI «National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V. I. Kulakov» of the Ministry of Health of the Russian Federation

Email: secretariat@oparina4.ru
ORCID iD: 0000-0003-4601-1330
117997, Moscow, Akademika Oparina St., 4

A. V. Asaturova

FSBI «National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V. I. Kulakov» of the Ministry of Health of the Russian Federation

Email: secretariat@oparina4.ru
ORCID iD: 0000-0001-8739-5209
MD, PhD, Head of first pathology Department 117997, Moscow, Akademika Oparina St., 4

G. R. Bayramova

FSBI «National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V. I. Kulakov» of the Ministry of Health of the Russian Federation

Email: secretariat@oparina4.ru
ORCID iD: 0000-0003-4826-661X
MD, PhD, of Medical Sciences, Honored Doctor of the Russian Federation, Professor of the Department of Obstetrics and Gynecology of the Department of Professional Education 117997, Moscow, Akademika Oparina St., 4

A. S. Badlaeva

FSBI «National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V. I. Kulakov» of the Ministry of Health of the Russian Federation

Email: secretariat@oparina4.ru
ORCID iD: 0000-0001-5223-9767
MD, PhD, Senior Researcher at the 1st Pathology Department 117997, Moscow, Akademika Oparina St., 4

A. V. Tregubova

FSBI «National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V. I. Kulakov» of the Ministry of Health of the Russian Federation

Email: secretariat@oparina4.ru
ORCID iD: 0000-0003-4601-1330
Pathologist 117997, Moscow, Akademika Oparina St., 4

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