Email this article Determination of antimicrobial activity of alcohol extractions of bark and buds of Quercus robur L.
- Authors: Ryabov N.A.1, Kurkin V.A.1, Ryzhov V.M.1, Lyamin A.V.1, Zhestkov A.V.1, Sokhina A.A.1
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Affiliations:
- Samara State Medical University
- Issue: Vol 20, No 1-2 (2020)
- Pages: 152-157
- Section: Pharmacy
- URL: https://ogarev-online.ru/2410-3764/article/view/54534
- DOI: https://doi.org/10.17816/2072-2354.2020.20.1.158-163
- ID: 54534
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Abstract
Antimicrobial activity of alcoholic extractions from the aboveground organs like bark and buds of Quercus robur L. was studied. The determination of the minimum inhibitory concentration (MIC) was performed with the use of double serial dilution in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. It has been revealed that alcoholic extractions of the bark of Quercus robur have the widest spectrum of antibacterial activity in relation to Pseudomonas aeruginosa, Escherichia coli and Candida albicans. Results of microbiological analysis show that alcoholic extractions from the bark and buds have an antimicrobial effect on all of these strains with a single dilution (except for antimicrobial activity against Staphylococcus aureus with 70% extractions of bark of Quercus robur). In particular, relatively high antimicrobial activity was observed in alcoholic extracts of all buds concentrations against Candida albicans at a dilution of 2, 4 and 8 times and Escherichia coli at a dilution of 4, 8 and 16 times. The greatest antimicrobial effect against Pseudomonas aeruginosa has extracts from the bark and buds in all the studied concentrations of alcohol. A significant antimicrobial effect against Escherichia coli has 40% of extractions from the buds of Quercus robur when diluted by 2, 4, 8, 16, 32 and 64 times. The results can be used as justification for the introduction of a new type of raw material that is buds of Quercus robur, as well as drugs based on it in the State Pharmacopoeia of the Russian Federation as antibacterial phytopharmaceuticals.
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##article.viewOnOriginalSite##About the authors
Nikolay A. Ryabov
Samara State Medical University
Author for correspondence.
Email: ryabov.nikolay.2014@mail.ru
Postgraduate student, Department of Pharmacognosy with Botany and Bases of Phytotherapy
Russian Federation, SamaraVladimir A. Kurkin
Samara State Medical University
Email: kurkinvladimir@yandex.ru
Doctor of Pharmaceutical Sciences, Professor, Head of the Department of Pharmacognosy with Botany and Bases of Phytotherapy
Russian Federation, SamaraVitaliy M. Ryzhov
Samara State Medical University
Email: lavr_rvm@mail.ru
Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Pharmacognosy with Botany and Bases of Phytotherapy
Russian Federation, SamaraArtem V. Lyamin
Samara State Medical University
Email: avlyamin@rambler.ru
Candidate of Medical Sciences, Associate Professor of the Department of General and Clinical Microbiology, Immunology and Allergology
Russian Federation, SamaraAlexander V. Zhestkov
Samara State Medical University
Email: avzhestkov2015@yandex.ru
Doctor of Medical Sciences, Professor, Head of the Department of General and Clinical Microbiology, Immunology and Allergology
Russian Federation, SamaraAnna A. Sokhina
Samara State Medical University
Email: annasohina381@gmail.com
Candidate of Pharmaceutical Sciences, Senior lecturer of the Department of Pharmaceutical Technology
Russian Federation, SamaraReferences
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