Email this article Techniques of quantitative evaluation of verapamil content in whole blood
- Authors: Voronin A.V.1
-
Affiliations:
- Samara State Medical University
- Issue: Vol 19, No 5-6 (2019)
- Pages: 116-121
- Section: Clinical Medicine
- URL: https://ogarev-online.ru/2410-3764/article/view/34213
- DOI: https://doi.org/10.17816/2072-2354.2019.19.3.116-121
- ID: 34213
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Abstract
Verapamil is a drug that can be toxic in pharmacological therapy and in case of misuse. Simple and informative methods of Verapamil quantification for forensic chemistry and hospital toxicology are needed.
Aim. The objective of the study was to compare analytical potential of different methods for Verapamil quantification used in forensic chemistry and hospital toxicology.
Materials and methods. The subject study was whole blood samples containing Verapamil. Verapamil in the blood samples was identified by gas chromatography-mass-spectrometry. Verapamil was quantified by thin-layer chromatography with videodensitometry and UV-spectrophotometry.
Results. To quantify Verapamil content, the plates were scanned, the chromatogram images were processed and calibration models were given by means of computer program. The calibration model is described by polynomial (square) regression. In UV-spectrophotometry absorbance of samples at the wavelength of 277 nm was measured; “blank” blood extracts as a zero reference were used. The limits of quantifation for thin-layer chromatography with videodensitometry and UV-spectrophotometry were 300,0 and 6000,0 ng/ml respectively. The accuracy and precision for thin-layer chromatography with videodensitometry failed to exceed 20.2 и 24.3% respectively; for UV-spectrophotometry they were 27.5 и 7.4%.
Conclusion. The ranges of quantifation make it possible to use thin-layer chromatography with videodensitometry for forensic chemistry and hospital toxicology. UV-spectrophotometry can be used to quantify Verapamil content in blood samples at the lethal concentration range.
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##article.viewOnOriginalSite##About the authors
Alexander V. Voronin
Samara State Medical University
Author for correspondence.
Email: dimmu2000@mail.ru
Candidate of Pharmaceutical Sciences, Associate Professor, Head of the Department of Chemistry of Pharmaceutical Faculty
Russian Federation, SamaraReferences
- Шорманов В.К., Квачахия Л.Л., Ртищев К.П. Определение верапамила в плазме крови // Курский научно-практический вестник «Человек и его здоровье». – 2014. – № 2. – С. 107–113. [Shormanov VK, Kvachahiya LL, Rtishchev KP. Verapamil determination in bloоd plasma. Kurskiy nauchno-prakticheskiy vestnik “Chelovek i ego zdorov’e”. 2014;(2):107-113. (In Russ.)]
- Мельников Е.С., Белова М.В., Родина Т.А., Раменская Г.В. Определение некоторых гипотензивных лекарственных веществ в плазме крови методом высокоэффективной жидкостной хроматографии/тандемной масс-спектрометрии при анализе острых отравлений // Сорбционные и хроматографические процессы. – 2015. – Т. 15. – № 2. – С. 257–268. [Mel’nikov ES, Belova MV, Rodina TA, Ramenskaya GV. Determination of some antihypertensive drugs in blood plasma by high performance liquid chromatography/tandem mass spectrometry in the analysis of acute poisonings. Sorbtsionnye i khromatograficheskie protsessy. 2015;15(2):257–268. (In Russ.)]
- Квачахия Л.Л., Шорманов В.К. Выявление верапамила в биологических жидкостях // Фармация. – 2015. – № 2. – С. 19–22. [Kvachakhia LL, Shormanov VK. Determination of verapamil in biological fluids. Pharmacy. 2015;(2):19–22. (In Russ.)]
- Gonzalez O, Alonso RM, Ferreirós N, et al. Development of an LC-MS/MS method for the quantitation of 55 compounds prescribed in combined cardiovascular therapy. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879(3-4):243-252. https://doi.org/10.1016/ j.jchromb.2010.12.007.
- Кормишин В.А. Денситометрическое определение некоторых накротических средств и психотропных веществ в химико-токсикологических исследованиях: Дис. … канд. фарм. наук. — Самара, 2014. [Kormishin VA. Densitometricheskoe opredelenie nekotoryh nakroticheskih sredstv i psihotropnyh veshhestv v himiko-toksikologicheskih issledovanijah. [dissertation] Samara; 2014. (In Russ.)]. Доступно по: http://www.dslib.net/farmakognozia/densitometricheskoe-opredelenie-nekotoryh-narkoticheskih-sredstv-i-psihotropnyh.html. Ссылка активна на 23.08.2019.
- Барсегян С.С., Саломатин Е.М., Плетенева Т.В., и др. Методические рекомендации по валидации аналитических методик, используемых в судебно-химическом и химико-токсикологическом анализе биологического материала. – М., 2014. – 73 с. [Barsegyan SS, Salomatin EM, Pleteneva TV, et al. Metodicheskie rekomendacii po validacii analiticheskih metodik, ispol’zuemyh v sudebno-himicheskom i himiko-toksikologicheskom analize biologicheskogo materiala. Moscow; 2014. 73 р. (In Russ.)]
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