Detection of Fungi in the Palmar Aponeurosis in Patients with Dupuytren’s Contracture by Scanning Electron Microscopy
- Authors: Stupina T.A.1, Migalkin N.S.1, Shchudlo N.A.1, Shchudlo M.M.1
-
Affiliations:
- Ilizarov National Medical Research Center of Traumatology and Orthopedics
- Issue: Vol 26, No 1 (2020)
- Pages: 147-152
- Section: RESEARCH METHODS
- URL: https://ogarev-online.ru/2311-2905/article/view/125108
- DOI: https://doi.org/10.21823/2311-2905-2020-26-1-147-152
- ID: 125108
Cite item
Abstract
The purpose of the study was to determine the capabilities of scanning electron and light microscopy in detecting fungi in surgical material from the patients with Dupuytren’s contracture. Material and Methods. The fragments of palmar aponeurosis from 27 patients with Dupuytren’s contracture were examined. Paraffin sections stained with hematoxylin-eosin and Periodic Schiff-Methenamine Silver were digitized using AxioScope.A1 microscope (Carl Zeiss Micro Imaging GmbH, Germany). For examination in JSM-840 scanning electron microscope (Jeol, Japan), the samples were dehydrated after fixation, soaked in camphene, dried, and silver sprayed in the IB-6 ionizer (Eiko, Japan). Results. In paraffin sections stained with methenamine-silver, the signs of mycotic microbiota were detected only in 2 (7.4%) of 27 patients, and with the use scanning electron microscopy — in 16 patients (59%). The morphological phenotypes and ultrastructure of intercellular contacts of yeast-like fungi were similar to Candida albicans. In all cases, the budding cells were detected. The destruction of the connective tissue of the palmar aponeurosis was observed in the form of loosening, stratification, and cavities formation (the zones of lysis). Conclusion. The scanning electron microscopy allowed to evaluate the ultrastructure of fungal cells and their interaction with surrounding tissues in the surgical material from the patients with Dupuytren’s contracture. A higher percentage of detection of fungal invasion compared to the Periodic Schiff-Methenamine staining was associated with a higher resolution and the ability of a larger area of tissue samples analysis.
About the authors
T. A. Stupina
Ilizarov National Medical Research Center of Traumatology and Orthopedics
Author for correspondence.
Email: StupinaSTA@mail.ru
Tatiana A. Stupina — Dr. Sci. (Biol.), Senior Researcher, Laboratory of Morphology
Kurgan
Russian FederationN. S. Migalkin
Ilizarov National Medical Research Center of Traumatology and Orthopedics
Email: fake@neicon.ru
Nikolai S. Migalkin — Researcher, Laboratory of Morphology
Kurgan
Russian FederationN. A. Shchudlo
Ilizarov National Medical Research Center of Traumatology and Orthopedics
Email: fake@neicon.ru
Natalia A. Shchudlo — Dr. Sci. (Med.), Head, Clinical and Experimental Laboratory of Reconstructive-Plastic Microsurgery and Hand Surgery
Kurgan
Russian FederationM. M. Shchudlo
Ilizarov National Medical Research Center of Traumatology and Orthopedics
Email: fake@neicon.ru
Mikhail M. Shchudlo — Dr. Sci. (Med.), Senior Researcher, Laboratory Clinical and Experimental Laboratory of Reconstructive and Plastic Microsurgery and Hand Surgery
Kurgan
Russian FederationReferences
- Ng M., Thakkar D., Southam L., Werker P., Ophoff R., Becker K., Nothnagel M. et al. A Genome-wide Association Study of Dupuytren Disease Reveals 17 Additional Variants Implicated in Fibrosis. Am J Hum Genet. 2017;101(3):417-427. doi: 10.1016/j.ajhg.2017.08.006.
- Unglaub F., Loos B., Schwarz S., Kneser U., Dragu A., Horch R.E. Phlegmonous-infection in first degree Dupuytren’s disease. Arch Orthop Trauma Surg. 2009;129(4):445-448. doi: 10.1007/s00402-008-0589-7.
- Dumitrescu-Ionescu D. A New Therapeutic Approach to Dupuytren’s Contracture / Disease (DD). Adv Plast Reconstr Surg. 2017;1(5):129-136.
- Guarner J., Brandt M.E. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev. 2011;24(2):247-280. doi: 10.1128/CMR.00053-10.
- Ensikat H.-J., Weigend M. Creating internal conductivity in dry biological SEM samples by a simple vapour treatment. J Microsc. 2014;256(3):226-230. doi: 10.1111/jmi.12177.
- Sugimoto S., Okuda K., Miyakawa R., Sato M., AritaMorioka K., Chiba A. et al. Imaging of bacterial multicellular behaviour in biofilms in liquid by atmospheric scanning electron microscopy. Sci Rep. 2016;6:25889. Available from: https://www.nature.com/articles/srep25889. doi: 10.1038/srep25889.
- Chebotar I.V., Novikov I.A., Subbot A.M., Mayansky N.A. Lanthanoid staining as a fast technology of preparing microbiological specimens for scanning electron microscopy. CTM. 2017;9(3):23-29. doi: 10.17691/stm2017.9.3.03.
- Raab N., Bachelet I. Resolving biofilm topography by native scanning electron microscopy. J Biol Methods. 2017;4(2):e70. doi: 10.14440/jbm.2017.173.
- Yue X., Wang A., Li Q. The Role of Scanning Electron Microscopy in the Direct Diagnosis of Onychomycosis. Scanning. 2018:1581495. doi: 10.1155/2018/1581495.
- Yuping R., Kaiwen Z., Wenying H., Jinghong H., Xiaowei F., Shuang C. et al. Observation of Fungi, Bacteria, and Parasites in Clinical Skin Samples Using Scanning Electron Microscopy. In: Modern Electron Microscopy in Physical and Life Sciences. 2016. Available from: https://www.intechopen.com/books/modernelectron-microscopy-in-physical-and-life-sciences/observation-of-fungi-bacteria-and-parasites-inclinical-skin-samples-using-scanning-electronmicrosc. doi: 10.5772/61850.
- Legge J.W., Finlay J.B., McFarlane R.M. A study of Dupuytren’s tissue with the scanning electron microscope. J Hand Surg Am. 1981;6(5):482-492. doi: 10.1016/s0363-5023(81)80109-8.
- Józsa L., Salamon A., Réffy A., Renner A., Demel S., Donhöffer A., Pintér T., Thöring J. Fine structural alterations of the palmar aponeurosis in Dupuytren’s contracture. A combined scanning and transmission electronmicroscopic examination. Zentralbl Allg Pathol. 1988;134(1):15-25.
- Щудло Н.А., Ступина Т.А., Щудло М.М. Сканирующая электронная микроскопия ладонного апоневроза при контрактуре Дюпюитрена продвинутой стадии. Травматология и ортопедия России. 2019;(25)2: 150-156. doi: 10.21823/2311-2905-2019-25-2-150-156.
- Tubiana R. Dupuytren’s disease of the radial side of the hand. Hand Clin. 1999;15(1):149-159.
- Scherer W.P., Scherer M.D. Scanning electron microscope imaging of onychomycosis. J Am Podiatr Med Assoc. 2004;94(4):356-362. doi: 10.7547/0940356.
- Kubota T. Chronic and recurrent vulvovaginal candidiasis. Anippon Isbink Gakkai Zassibi. 1998;(39)4: 213-218. doi: 10.3314/jjmm.39.213.
- Хмельницкий О.К., Быков В.Л., Хмельницкая Н.М. Патоморфологическая диагностика микозов, вызываемых условно-патогенными грибами. Режим доступа: http://www.rusmedserv.com/mycology/html/labora15.htm
- Staniszewska M., Bondaryk M., Swoboda-Kopec E., Siennicka K., Sygitowicz G., Kurzatkowski W. Candida albicans morphologies revealed by scanning electron microscopy analysis. Braz J Microbiol. 2013;44(3):813-821. doi: 10.1590/S1517-83822013005000056.
- Wade R., Igali L., Figus A. Skin involvement in Dupuytren’s disease. J Hand Surg Eur. 2016;41(6):600-608. doi: 10.1177/1753193415601353.
- Щудло Н.А., Костин В.В. Патогенез н ропатии при контрактуре Дюпюитрена. Гений ортопедии. 2019;25(1):58-64. doi: 10.18019/1028-4427-2019-25-1-58-64.
- Долганова Т.И., Щудло Н.А., Шабалин Д.А., Костин В.В. Оценка гемодинамики артерий кисти и микроциркуляции кожи при контрактуре Дюпюитрена 3-4 стадий до и после оперативного лечения с применением чрескостной фиксации по Г.А. Илизарову. Гений ортопедии. 2019;25(1):86-92. doi: 10.18019/1028-4427-2019-25-1-86-92.
- Rehman S., Goodacre R., Day P.J., Bayat A., Westerhoff H.V. Dupuytren’s: a systems biology disease. Arthritis Res Ther. 2011;13(5):238. doi: 10.1186/ar3438.
- Badiee P., Hashemizadeh Z. Opportunistic invasive fungal infections: diagnosis & clinical management. Indian J Med Res. 2014;139(2):195-204.
- Gudmundsson K.G., Arngrímsson R., Sigfússon N., Jónsson T. Increased total mortality and cancer mortality in men with Dupuytren’s disease: a 15-year follow-up study. J Clin Epidemiol. 2002;55(1):5-10. doi: 10.1016/s0895-4356(01)00413-9.
Supplementary files
