Prospects of using melanin as an antimicrobial agent in food packaging

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Abstract

Melanin, known for its high physiological activity and functional properties, has attracted research interest as a potential antimicrobial agent in food systems and consumer packaging. This study investigated the antimicrobial activity of melanin derived from buckwheat hulls against the bacterial microbiota present in industrially produced cheeses. The research objects were purified melanin obtained from buckwheat hulls and the following microbial strains: Penicillium roqueforti, Bacillus subtilis, Bacillus pumilus, and Lactobacillus plantarum. The disc-diffusion method and the cultivation of test strains in the presence of melanin were employed. Statistical analysis was carried out using Statistica 10 software. The study confirmed the antimicrobial activity of melanin against Gram-positive bacteria Bacillus subtilis B-12587 and Penicillium roqueforti F-1311. At the highest tested concentration, the zone of lysis for Bacillus subtilis B-12587 was 26.4±0.2 mm, which was 1.28 times larger than that of Penicillium roqueforti F-1311 cells, 1.43 times larger than that of Bacillus pumilus B-7308 cells, and 1.58 times larger than that of Lactobacillus plantarum B-3242 cells. A strong correlation (r = 0.97) was identified between melanin concentration in the medium and the growth inhibition of Bacillus subtilis B-12587. These findings highlight the potential of plant-derived melanins as antimicrobial agents in food-grade films intended for primary packaging.

About the authors

Ya. V. Urazova

Biysk Technological Institute (branch) of the Altai State Technical University

Email: urazova.iav@bti.secna.ru
ORCID iD: 0000-0002-6847-8487

E. D. Rozhnov

Ural State University of Economics

Email: red@bti.secna.ru
ORCID iD: 0000-0002-3982-9700

M. N. Shkolnikova

Ural State University of Economics

Email: shkolnikova.m.n@mail.ru
ORCID iD: 0000-0002-9146-6951

References

  1. Кадрицкая Е.А., Школьникова М.Н. Применение меланина в пищевой промышленности // Аграрно-пищевые инновации. 2022. № 3. С. 69–76. doi: 10.31208/2618-7353-2022-19-69-76. EDN: TKHXRP.
  2. Mostert A.B. Melanin, the what, the why and the how: An introductory review for materials scientists interested in flexible and versatile polymers // Polymers. 2021. Vol. 13, no. 10. P. 1670. doi: 10.3390/polym13101670.
  3. Lomovskiy I., Kiryanov A., Skripkina T. The effect of reverse sorption on an extraction kinetics melanin case // Processes. 2023. Vol. 11, no. 11. P. 3192. doi: 10.3390/pr11113192.
  4. Minasyan E., Aghajanyan A., Karapetyan K., Khachaturyan N., Hovhannisyan G., Yeghyan K., et al. Antimicrobial activity of melanin isolated from wine waste // Indian Journal of Microbiology. 2024. Vol. 64. P. 1528–1534. doi: 10.1007/s12088-023-01155-9.
  5. Tsouko E., Tolia E., Sarris D. Microbial melanin: renewable feedstock and emerging applications in food-related systems // Sustainability. 2023. Vol. 15, no. 9. P. 7516. doi: 10.3390/su15097516.
  6. Yang M., Li L., Yu S., Liu J., Shi J. High performance of alginate/polyvinyl alcohol composite film based on natural original melanin nanoparticles used as food thermal insulating and UV-vis block // Carbohydrate Polymer. 2020. Vol. 233. P. 115884. doi: 10.1016/j.carbpol.2020.115884.
  7. Bang Y.-J., Shankar S., Rhim J.-W. Preparation of polypropylene/poly (butylene adipate-co-terephthalate) composite films incorporated with melanin for prevention of greening of potatoes // Packaging Technology and Science. 2020. Vol. 33, no. 10. P. 433–441. https://doi.org/10.1002/pts.2525.
  8. Łopusiewicz L., Drozlowska E., Trocer P., Kostek M., Śliwiński M., Henriques M.H.F., et al. Whey protein concentrate/isolate biofunctional films modified with melanin from watermelon (Citrullus lanatus) seeds // Materials. 2020. Vol. 13, no. 17. P. 3876. doi: 10.3390/ma13173876.
  9. Liang Y., Zhao Y., Sun H., Dan J., Kang Y., Zhang Q., et al. Natural melanin nanoparticle-based photothermal film for edible antibacterial food packaging // Food Chemistry. 2023. Vol. 401. P. 134117. doi: 10.1016/j.foodchem.2022.134117.
  10. Łopusiewicz Ł., Jędra F., Mizielińska M. New poly(-lactic acid) active packaging composite films incorporated with fungal melanin // Polymers. 2018. Vol. 10, no. 4. P. 386. doi: 10.3390/polym10040386.
  11. Roy S., Rhim J.-W. Preparation of carrageen-an-based functional nanocomposite films incorporated with melanin nanoparticles // Colloids and Surfaces B: Biointerfaces. 2019. Vol. 176. P. 317–324. doi: 10.1016/j.colsurfb.2019.01.023.
  12. Roy S., Shankar S., Rhim J.-W. Melanin-mediated synthesis of silver nanoparticle and its use for the preparation of carrageenan-based antibacterial films // Food Hydrocolloids. 2019. Vol. 88. P. 237–246. doi: 10.1016/j.foodhyd.2018.10.013.
  13. Kiran G.S., Dhasayan A., Lipton A.N., Selvin J., Arasu M.V., Al-Dhabi N.A. Melanin-templated rapid synthesis of silver nanostructures // Journal of Nanobiotechnology. 2014. Vol. 12, no. 1. P. 18. doi: 10.1186/1477-3155-12-18.
  14. Galaby S.S., Maharik N.M.S., Khalifa M.I. Prevalence of some deteriorating microorganisms in raw milk and some locally made cheese // New Valley Veterinary Journal. 2021. Vol. 1, no. 2. P. 21–27. doi: 10.21608/nvvj.2021.205838.
  15. Ibrahim R.A., El-Salam B.A.A., Alsulami T., Ali H.S., Hoppe K., Badr A.N. Neoteric biofilms applied to enhance the safety characteristics of Ras cheese during ripening // Foods. 2023. Vol. 12, no. 19. P. 3548. doi: 10.3390/foods12193548.
  16. Ferraz A.R., Goulão M., Santo C.E., Anjos O., Serralheiro M.L., Pintado C.M.B.S. Novel, edible melanin-protein-based bioactive films for cheeses: antimicrobial, mechanical and chemical characteristics // Foods. 2023. Vol. 12, no. 9. P. 1806. doi: 10.3390/foods12091806.
  17. Школьникова М.Н., Аверьянова Е.В., Рожнов Е.Д., Баташов Е.С. Исследование антибактериальной активности флавоноидов облепихового шрота // Индустрия питания. 2020. Т. 5. N 3. С. 61–69. doi: 10.29141/2500-1922-2020-5-3-7. EDN: AWZINX.
  18. Пат. № 2780731, Российская Федерация, МПК C09B 61/00. Способ выделения меланина из лузги гречихи / Я.В. Уразова, Е.Д. Рожнов, Л.А. Бахолдина, Е.А. Кадрицкая, М.Б. Ребезов, М.А. Шариати. Заявл. 02.06.2021; опубл. 29.09.2022. Бюл. № 28.
  19. Zhang Z., Yang Y., Xi H., Yu Y., Song Y., Wu C., et al. Evaluation methods of inhibition to microorganisms in biotreatment processes: a review // Water Cycle. 2023. Vol. 4. P. 70–78. doi: 10.1016/j.watcyc.2023.02.004.
  20. Nielsen S.L., Hansen B.W. Evaluation of the robustness of optical density as a tool for estimation of biomass in microalgal cultivation: The effects of growth conditions and physiological state // Aquaculture Research. 2019. Vol. 50, no 9. P. 2698–2706. doi: 10.1111/are.14227.

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