Evaluation of antifungal and antioxidant activities of extracts prepared from earthworm (Perionyx excavates) using different solvents
- 作者: Dung N.T.1, Son P.H.1, Trinh N.T.1, Thao V.N.1, Tu B.L.1, Luan L.Q.1, Thinh B.B.1, Hai L.V.1,2
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隶属关系:
- Biotechnology Center of Ho Chi Minh City
- Phan Chau Trinh University
- 期: 卷 14, 编号 4 (2024)
- 页面: 462-471
- 栏目: Physico-chemical biology
- URL: https://ogarev-online.ru/2227-2925/article/view/302271
- DOI: https://doi.org/10.21285/achb.953
- EDN: https://elibrary.ru/HZYTZA
- ID: 302271
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详细
This study evaluated the antifungal and antioxidant activities of earthworm (Perionyx excavatus) powder extracts using different solvents including 80% ethanol, 50% ethanol, and distilled water. The extraction efficiencies ranged from 18.5 to 21.2%, while total protein contents ranged from 64.8 to 67.5%. Notably, the aqueous extract exhibited the highest values in both extraction efficiency and total protein content. Thin-layer chromatography analysis revealed the presence of amino acids and peptides with Rf values ranging from 0.42 to 0.65. Fourier-transform infrared spectroscopy spectra displayed characteristic peaks associated with protein structures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated protein compositions primarily below 50 kDa molecular weight in the extracts, particularly with the 80% ethanol extract predominantly consisting of proteins below 30 kDa. Antifungal tests against Candida albicans demonstrated the highest efficacy with 80% ethanol extract, exhibiting an inhibition zone diameter of 13.3 mm and a minimum inhibitory concentration of 75 mg/mL. Additionally, the extracts showed DPPH and ABTS radical scavenging activities, with 80% ethanol extract displaying the highest antioxidant potential with IC50 values of 231.3 μg/mL for DPPH assay and 208.9 μg/mL for ABTS assay. In conclusion, earthworm powder extracts exhibited significant biological activities, rendering them promising candidates for pharmaceutical and cosmetic applications.
作者简介
N. Dung
Biotechnology Center of Ho Chi Minh City
编辑信件的主要联系方式.
Email: thuydung9810@gmail.com
Ph. Son
Biotechnology Center of Ho Chi Minh City
Email: haison010695@gmail.com
N. Trinh
Biotechnology Center of Ho Chi Minh City
Email: nutrinh28218@gmail.com
V. Thao
Biotechnology Center of Ho Chi Minh City
Email: vothao2297@gmail.com
B. Tu
Biotechnology Center of Ho Chi Minh City
Email: khatu09021990@gmail.com
L. Luan
Biotechnology Center of Ho Chi Minh City
Email: lequangluan@gmail.com
B. Thinh
Biotechnology Center of Ho Chi Minh City
Email: buibaothinh9595@gmail.com
L. Hai
Biotechnology Center of Ho Chi Minh City ; Phan Chau Trinh University
Email: hailevan.chem@gmail.com
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