Heterogeneity of virulence factors among Porphyromonas gingivalis clinical isolates from patients with chronic generalized periodontitis

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Abstract

The development of chronic generalized periodontitis occurs due to a combination of a several causes, among which the leading role is assigned to periodontal pathogens, which include P. gingivalis. Among P. gingivalis virulence factors, the polysaccharide capsule, fimbria proteins, cysteine proteases, and hemagglutinins are of special importance. The study was aimed to investigate the prevalence of specific virulence genes and identify a virulent genotype among P. gingivalis isolates found in patients with severe chronic generalized periodontitis (CGP). 41 patients (27 women and 14 men, average age 43.9±1.5 years) were examined, of which main and control group consisted of 22 patients with severe CGP and 19 patients without inflammatory periodontal diseases, respectively. The PCR data allow to consider type II fimbria (FimA II), arginine-dependent type A protease (RghA) and lysine-dependent protease (Kgh) as specific markers for the detection of more virulent P. gingivalis strains. It was found that in St. Petersburg, the following P. gingivalis genotypes predominate among patients with severe CGP: fimA II:kg:rghA, fimA II:kgh and fimA II:rghA. In addition, it has been demonstrated that virulent genotypes are detected to a small extent in P. gingivalis isolates from healthy control group patients. The identification of P. gingivalis strains with a more prominent pathogenic potential and the detection of their virulent genotypes is of great practical importance, in the future allowing to develop advanced effective methods for disease prevention to be used in a personalized medicine strategy. The results obtained are also of high importance due to the recorded variability in the circulation of P. gingivalis strain genotypes in various worldwide regions.

About the authors

I. V. Koroleva

St. Petersburg State University; Institute of Experimental Medicine

Email: e.michailova@spbu.ru

PhD (Biology), Associate Professor, Department of Fundamental Problems of Medicine and Medical Technologies; Senior Researcher, Department of Molecular Microbiology

Russian Federation, St. Petersburg; St. Petersburg

Ekaterina S. Mikhailova

St. Petersburg State University

Author for correspondence.
Email: e.michailova@spbu.ru

DSc (Medicine), Associate Professor, Associate Professor of the Department of Therapeutic Dentistry

Russian Federation, St. Petersburg

K. A. Privalova

Pavlov First St. Petersburg State Medical University

Email: e.michailova@spbu.ru

Resident of the Department of Oral and Maxillofacial Surgery and Surgical Dentistry

Russian Federation, St. Petersburg

L. A. Ermolaeva

St. Petersburg State University

Email: e.michailova@spbu.ru

DSc (Medicine), Professor, Head of the Therapeutic Dentistry Department

Russian Federation, St. Petersburg

S. A. Tumanova

St. Petersburg State University

Email: e.michailova@spbu.ru

PhD (Medicine), Associate Professor, Associate Professor of the Department of Therapeutic Dentistry

Russian Federation, St. Petersburg

A. N. Suvorov

St. Petersburg State University; Institute of Experimental Medicine

Email: e.michailova@spbu.ru

RAS Corresponding Member, DSc (Medicine), Professor, Head of the Fundamental Problems of Medicine and Medical Technologies Department; Head of the Molecular Microbiology Department

Russian Federation, St. Petersburg; St. Petersburg

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Supplementary files

Supplementary Files
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2. Figure 1. Complaints from patients in the main and control groups

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3. Figure 2. OHI-S, CPITN, Silness-Loe, PMA (%) и BOP (%) index rates in patients of the main and control groups

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4. Figure 3. Frequency of P. gingivalis occurrence in periodontal pockets/gingival sulcus in patients of the main and control groups

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5. Figure 4. Prevalence of fimbriae types I, II and IV genes (fimA I, fimA II and fimA IV) among P. gingivalis isolates in patients of the main and control groups

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6. Figure 5. Prevalence of lysine-dependent protease (kgh) and arginine-dependent protease type A (rghA) genes among P. gingivalis isolates in patients of the main and control groups

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Copyright (c) 2024 Koroleva I.V., Mikhailova E.S., Privalova K.A., Ermolaeva L.A., Tumanova S.A., Suvorov A.N.

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