DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
- Authors: Dukhovlinov I.V.1, Bogomolova E.G.1, Fedorova E.A.1, Simbirtsev A.S.1
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Affiliations:
- Research Institute of Highly Pure Biopreparations, St. Petersburg
- Issue: Vol 4, No 3 (2014)
- Pages: 229-234
- Section: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/2220-7619/article/view/118402
- DOI: https://doi.org/10.15789/2220-7619-2014-3-229-234
- ID: 118402
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Abstract
Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for prevention of rotavirus infection. However attenuated alive vaccines are used for this purpose which can result in different complications. The candidate vaccine presented in this study was designed on the basis of recombinant fusion proteins — the crucial active agents for development of immune response against rotaviruses. As part of the study the following steps were taken: the design of fusion proteins VP6VP8 and FliCVP6VP8; protocol adjustment for induction of genes encoding these proteins expression, which yields in high level of the protein with minor expenditures; production and purification of recombinant proteins VP6VP8 and FliCVP6VP8 with metal affinity chromatography.
About the authors
I. V. Dukhovlinov
Research Institute of Highly Pure Biopreparations, St. Petersburg
Author for correspondence.
PhD (Biology), Head of the Laboratory of Genetic Engineering of Vaccines, Research Institute of Highly Pure Biopreparations Russian Federation
E. G. Bogomolova
Research Institute of Highly Pure Biopreparations, St. PetersburgJunior Researcher, Laboratory of Genetic Engineering of Vaccines, Research Institute of Highly Pure Biopreparations Russian Federation
E. A. Fedorova
Research Institute of Highly Pure Biopreparations, St. PetersburgResearcher, Laboratory of Genetic Engineering of Vaccines, Research Institute of Highly Pure Biopreparations Russian Federation
A. S. Simbirtsev
Research Institute of Highly Pure Biopreparations, St. Petersburg
Email: simbirtsev@hpb-spb.com
PhD, MD (Medicine), Director of the Research Institute of Highly Pure Biopreparations Russian Federation
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