Enteric nervous system with chronic mesenteric ischemia: experimental study

Evgeniya S. Pimenova; Пименова Евгения Сергеевна; Natal′ya S. Korchagina; Корчагина Наталья Сергеевна; Grigoriy A. Korolev; Королев Григорий Алексеевич; Dar′ya D. Zyuz′ko; Зюзько Дарья Дмитриевна; Margarita S. Saakyan; Саакян Маргарита Степановна; Dmitriy A. Morozov; Морозов Дмитрий Анатольевич

doi:10.17816/psaic703

Enteric nervous system with chronic mesenteric ischemia: experimental study

作者: Pimenova E.S.¹, Korchagina N.S.², Korolev G.A.¹, Zyuz′ko D.D.¹, Saakyan M.S.³, Morozov D.A.¹
隶属关系:
1. I.M. Sechenov First Moscow State Medical University (Sechenov University)
2. Filatov Сhildren’s Hospital
3. Central State Medical Academy of Department of Presidential Affairs Administrative Department of the President of the Russian Federation
期: 卷 10, 编号 4 (2020)
页面: 401-410
栏目: Original Study Articles
URL: https://ogarev-online.ru/2219-4061/article/view/122937
DOI: https://doi.org/10.17816/psaic703
ID: 122937

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Introduction. The pathology of the enteric ganglia can lead to different diseases (Hirschsprung’s, neuronal intestinal dysplasia, ganglioneuromatosis, and Chagasse). Causes of acquired dysganglionosis remained unclear. Some authors hypothesized that pathology of the enteral nervous system may be secondary to intestinal ischemia.

Aim. To investigate the intestinal function and histological changes of the colon in rats with chronic ischemia.

Materials and methods. A total of 20 Sprague Dawley rats underwent surgery (ligation of the terminal mesenteric vessels next to the descending colon). The appetite of animals were checked, and stool were collected after the procedure. Reoperation was performed after 7 (n = 1), 9 (n = 2), 12 (n = 2), 14 (n = 1), 21 (n = 1), 42 (n = 1), 53 (n = 1), and 62 (n = 1) days. The diameter of the colon and changes of the serosa were visualized. In the experimental group, two samples biopsy was performed (ischemic and normal colon).

Results. Functional changes were observed in 90% of rats after the ligation of mesenteric vessels (constipation/impact, softening stool/diarrhea, and hemocolitis). Colonic stenosis of the ischemic area in 30% was detected. 70% animals have the intestinal dilatation above the ischemic segment (partial bowel obstruction). Necrosis of the ischemic colon was observed in 20%. Spontaneous fixation of the omentum to the ischemic segment was found in 40% animals. A microscopically inflamed infiltration of the mucosa in the ischemic zone (70%) and in normal colon (50%) was revealed in the ligation group. The number of the enteric ganglia decreased in the ischemic segment.

Conclusion. Functional disorders (colitis and obstruction) and morphological changes (inflammation and ganglion cells pathology) were found in rats with chronic mesenteric ischemia.