The neuroprotective action of endocannabinoid N-arachidonoyldopamine (AA-DA) in the model of apoptosis induced in cultured rat cerebellar granule neurons by K+ deprivation was investigated. The efficacy of simultaneous application of AA-DA with fatty acid amide hydrolase inhibitor N-eicosapentaenoyldopamine (EPA-5HT) was also evaluated. It was shown that AA-DA dose-dependently protects neurons, with maximal effect of 65% survived cells observed at concentration 10 μM. The use of submaximal concentration (5 μM) of AA-DA in combination with EPA-5HT (10 μM) led to significant enhancement of neuroprotection. The value of cell survival for the combination exceeded such values for AA-DA alone in concentration of 5 and 10 μM, increasing the neuronal survival up to 78%. It may be concluded that such combination allows to reduce efficient concentration of neuroprotector and to diminish its possible side effects. Thus, the simultaneous application of endocannabinoid system modulators such as cannabinoid receptor agonists and inhibitors of endocannabinoid hydrolysis appears to be effective approach for neural cells protection.
Modulators of endogenous cannabinoid system аs neuroprotectors
- Authors: Genrikhs E.E.1, Bobrov M.Y.2, Andrianova E.L.1, Gretskaya N.M.2, Lyzhin A.A.1, Blazhenova A.V.2, Frumkina L.E.1, Bezuglov V.V.2, Khaspekov L.G.1
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Affiliations:
- Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (Moscow)
- Issue: Vol 4, No 4 (2010)
- Pages: 37-42
- Section: Original articles
- URL: https://ogarev-online.ru/2075-5473/article/view/124475
- DOI: https://doi.org/10.17816/psaic323
- ID: 124475
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About the authors
E. E. Genrikhs
Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
Author for correspondence.
Email: platonova@neurology.ru
Russian Federation
M. Yu. Bobrov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
E. L. Andrianova
Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
N. M. Gretskaya
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
A. A. Lyzhin
Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
A. V. Blazhenova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
L. E. Frumkina
Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
V. V. Bezuglov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
L. G. Khaspekov
Research Center of Neurology, Russian Academy of Medical Sciences (Moscow)
Email: platonova@neurology.ru
Russian Federation
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