Possibilities of optimization of transfusion therapy in gastrosurgery

Objective. To develop techniques pretransfusion rehabilitation canned red blood cells in patients with perioperative blood loss, severe. Materials and methods. The object of the study served as the red cells of various human blood storage time, stable preservative CPDA-1. Ozonation red blood cells was carried out by mixing it with ozonated solution NaCl 0.9% in an equivalent volume containing various concentrations of ozone, discrete increases of up to 20 mg/l. After 30 minutes of exposure to the slurry obtained erythrocyte suspension was determined concentration of malondialdehyde, adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), catalase activity in erythrocytes and their electrophoretic mobility. Results of the study. Under the influence of different ratios of ozone and erythrocytes detected ozone concentration range of 0.5-2 mg/l, causing an equivalent mixing restoration of ATP levels and 2,3-DPG in red blood cells to near normal values during the shelf life of 7 to 21 days. In terms of storage of packed red blood cells to 30 days, an increase 2,3-DPG content in red blood cells was observed on the background of the depletion of ATP pools in the cells. Thus inside the erythrocytes catalase activity in the basic group increases, resulting in 30 minutes after treatment, a significant reduction of malondialdehyde, without disturbing the electrophoretic mobility of treated erythrocytes. Conclusion. The work demonstrated the potential effects of ozone at low concentrations on key factors such as the system of oxygen transport and energy metabolic status of erythrocytes, as well as their morphological and functional properties (primarily deformability) at pretransfusion processing.

2, canned red cells, ozone, adenosine triphosphate, 2, 3-diphosphoglycerate, malondialdehyde