The Effect of Oncolytic Reovirus P-92 on Dendritic Cell Maturation and Generation of Tumor-Specific Lymphocytes in vitro

Resumo

Utilization of oncolytic viruses (OVs) is a promising approach to inhibit the tumor growth. OVs exhibit both direct antitumor and indirect immunostimulatory effects. It is known that the recognition of tumor antigen by cells of the immune system is disturbed at cancer pathologies. Stimulation of dendritic cells (DCs) with tumor antigens together with viruses may enhance the generation of antigen-specific lymphocytes against tumor cells, which in turn can be an effective tool for antitumor cell technologies. The aim of this study was to clarify the possibility of using reovirus P-92 for the generation of tumor-specific T-lymphocytes in vitro. We found that the differentiation of DCs in the presence of reovirus P-92 increased the yield of mature DCs. In addition, DCs with loaded HeLa antigens after cocultivation with lymphocytes and HeLa cells did not demonstrate an active cytolytic activity. Only a cytostatic effect was observed, unlike in the case of samples with the added reovirus P-92. Thus, the antigen, which is HeLa cells cultured with reovirus P-92 under the conditions we described, can be proposed as an effective experimental product for the cellular cancer immunotherapy.