Comparative Studies of Glutamine Synthetase Levels in the Brains of Patients with Schizophrenia and Mentally Healthy People
- Authors: Boksha I.S.1, Tereshkina E.B.1, Savushkina O.K.1,2, Prokhorova T.A.1, Vorobyeva E.A.1, Burbaeva G.S.1
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Affiliations:
- Mental Health Research Center
- Aff2
- Issue: Vol 12, No 1 (2018)
- Pages: 95-101
- Section: Clinical Neurochemistry
- URL: https://ogarev-online.ru/1819-7124/article/view/211449
- DOI: https://doi.org/10.1134/S1819712418010026
- ID: 211449
Cite item
Abstract
In this study the authors compared the levels of glutamine synthetase (GS) enzymatic activities, as well as the levels of immunoreactive GS and GS-like protein (GSLP) in the readily soluble protein fraction of brain samples taken during autopsies of patients with schizophrenia and subjects from a control group. Enzymatic GS activity was measured and levels of immunoreactive GS and GSLP were determined in postmortem samples of the prefrontal, anterior, and posterior cingulate cortex (Brodmann areas 10, 24, and 23, respectively) and cerebellum cortex obtained from patients with schizophrenia (n = 8) and a control group (n = 9), matched according to age and postmortem interval. GS activity was evaluated in vitro in the “transferase” reaction by the formation of γ-glutamyl hydroxamate, and the levels of immunoreactive GS and GSLP were evaluated by ECL Western blotting using monoclonal and polyclonal antibodies. No differences in GS enzymatic activity were found in all of the studied brain structures. The level of immunoreactive GS in the prefrontal cortex was significantly decreased in patients with schizophrenia compared with the controls (at p < 0.001 according to the Mann–Whitney U-test); it was unchanged in the posterior cingulate cortex and significantly elevated in the anterior cingulate cortex and cerebellum (p < 0.001 and p < 0.004, respectively), whereas the level of immunoreactive GSLP was elevated in all studied brain areas (p < 0.004, p < 0.02, p < 0.04, and p < 0.006, respectively). The alteration of GS and GSLP levels in brains of patients with schizophrenia is one of the factors that disturb glutamate metabolism in these brain structures and is an important aspect of schizophrenia pathogenesis.
About the authors
I. S. Boksha
Mental Health Research Center
Email: neurochem06@mail.ru
Russian Federation, Moscow
E. B. Tereshkina
Mental Health Research Center
Email: neurochem06@mail.ru
Russian Federation, Moscow
O. K. Savushkina
Mental Health Research Center; Aff2
Author for correspondence.
Email: neurochem06@mail.ru
Russian Federation, Moscow; Zagorodnoe sh. 2 str. 16, Moscow, 117152
T. A. Prokhorova
Mental Health Research Center
Email: neurochem06@mail.ru
Russian Federation, Moscow
E. A. Vorobyeva
Mental Health Research Center
Email: neurochem06@mail.ru
Russian Federation, Moscow
G. Sh. Burbaeva
Mental Health Research Center
Email: neurochem06@mail.ru
Russian Federation, Moscow
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