Melatonin Modulates Phosphorylation of 2′,3′-Cyclic Nucleotide-3′-Phosphodiesterase in the Presence of Protoporphyrin IX in the Brain Mitochondria of Rats during the Functioning of the Non-Specific Mitochondrial Pore
- Авторы: Krestinina O.V.1,2, Baburina Y.L.1, Odinokova I.V.1, Azarashvili T.S.1, Akatov V.S.1
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Учреждения:
- Institute of Theoretical and Experimental Biophysics
- Aff2
- Выпуск: Том 12, № 1 (2018)
- Страницы: 33-40
- Раздел: Experimental Articles
- URL: https://ogarev-online.ru/1819-7124/article/view/211403
- DOI: https://doi.org/10.1134/S1819712418010051
- ID: 211403
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Аннотация
Melatonin (N-acetyl-5-methoxytryptamine), a neuroendocrine hormone of the pineal gland, participates in the modulation of the mitochondrial nonspecific pore (mitochondrial permeability transition pore, mPTP). According to the results we obtained, protoporphyrin IX (PPIX, a ligand of the TSPO translocator protein) induces the opening of the mPTP in brain mitochondria; melatonin slows the induction of the mPTP in rat brain mitochondria incubated with PPIX. Induction of the mPTP activates protein kinases/protein phosphatases that are involved in the regulation of protein phosphorylation. The TSPO modulates protein phosphorylation; in the presence of PPIX, the phosphorylation of 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) increases. Here, we showed an increase in the degree of phosphorylation of CNPase in PPIX-treated rat brain mitochondria, a melatonin-induced decrease in level of CNPase phosphorylation in mitochondria incubated with PPIX; hence, we propose that melatonin participates in TSPOmodulated protein phosphorylation.
Об авторах
O. Krestinina
Institute of Theoretical and Experimental Biophysics; Aff2
Автор, ответственный за переписку.
Email: ovkres@mail.ru
Россия, Pushchino; ul. Institutskaya 3, Pushchino, Moscow oblast, 142290
Yu. Baburina
Institute of Theoretical and Experimental Biophysics
Email: ovkres@mail.ru
Россия, Pushchino
I. Odinokova
Institute of Theoretical and Experimental Biophysics
Email: ovkres@mail.ru
Россия, Pushchino
T. Azarashvili
Institute of Theoretical and Experimental Biophysics
Email: ovkres@mail.ru
Россия, Pushchino
V. Akatov
Institute of Theoretical and Experimental Biophysics
Email: ovkres@mail.ru
Россия, Pushchino
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