Levels of GFAP, Iba1, TNF-α, and caspase-3 in the cingulate cerebral cortex of mice after intraperitoneal lipopolysaccharide

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Abstract

Introduction. Bacterial lipopolysaccharide (LPS) affects the structure and function of nervous tissue cells.

The aim of the study: to explore the impact of intraperitoneal LPS given to mice on levels of such markers as glial fibrillar acidic protein (GFAP), adapter protein binding ionized calcium (Iba1), tumor necrosis factor alpha (TNF-α), and caspase-3 in the cingulate cerebral cortex.

Material and methods. C57Bl/6 mice aged 3 months were injected with saline solution (control group) or escherichial LPS at a dose of 1 mg/kg of body mass (comparison group) once a day for 4 consecutive days. On Day 5, the animals were sacrificed with further coronal sections of the brain of 5 µm done and staining provided with immunofluorescent antibodies to GFAP, Iba1, TNF-α, and caspase-3.

Results. Animals of the comparison group had the number of GFAP- and Iba1-positive cells in the cingulate cortex that was significantly larger than in animals of the control group. The area of the ground substance with a positive antibody reaction to TNF-α in the mice of the comparison group stayed increased, and the area of the cytoplasm with a positive antibody reaction to caspase-3 did not differ between the groups.

Conclusion. The revealed levels for GFAP and IBA1 markers with the increased content of TNF-α in the ground substance of the nervous tissue without changes in the levels of caspase-3 in the cytoplasm of cells suggest a significant role of TNF-α release in the reaction of neuroglia to LPS. It is necessary to further study the morphological changes in the nervous tissue in the applied mode of administration of LPS.

About the authors

Artem A. Venediktov

Sechenov First Moscow State Medical University (Sechenov University)

Email: venediktov_a_a@staff.sechenov.ru
ORCID iD: 0000-0002-5604-0461
SPIN-code: 4791-1926

PhD Student, Assistant Professor at Human Anatomy and Histology Department

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

Egor A. Kuzmin

Sechenov First Moscow State Medical University (Sechenov University)

Email: kuzmin.egor.msmu@yandex.ru
ORCID iD: 0000-0003-4098-1125
SPIN-code: 1835-6549

Research Intern, Human Anatomy and Histology Department

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

Ksenia S. Pokidova

Sechenov First Moscow State Medical University (Sechenov University)

Email: pokidowa.ks@gmail.com
ORCID iD: 0009-0000-0323-7669
SPIN-code: 4016-5570

Research Intern

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

David M. Oganesyan

Sechenov First Moscow State Medical University (Sechenov University)

Email: oganesyan_d_m@student.sechenov.ru
ORCID iD: 0009-0005-2537-8370

4th Year Student

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

Arina T. Stepanyan

Sechenov First Moscow State Medical University (Sechenov University)

Email: stepanyan_a_t@student.sechenov.ru
ORCID iD: 0009-0004-5771-4749

4th Year Student

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

Tatiana V. Boronikhina

Moscow University for Industry and Finance “Synergy”

Author for correspondence.
Email: tvbor51@mail.ru
ORCID iD: 0000-0001-9532-7898
SPIN-code: 3544-9503

Doctor of Medical Sciences, Professor, Biomedical Department

Russian Federation, Meschanskaya str., 9/14, 1, Moscow, 129090

Gennadii A. Piavchenko

Sechenov First Moscow State Medical University (Sechenov University)

Email: gennadii.piavchenko@staff.sechenov.ru
ORCID iD: 0000-0001-7782-3468
SPIN-code: 6120-1057

Candidate of Medical Sciences, Associate Professor, Human Anatomy and Histology Department

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

Sergey L. Kuznetsov

Sechenov First Moscow State Medical University (Sechenov University)

Email: kuznetsov_s_l@staff.sechenov.ru
ORCID iD: 0000-0002-0704-1660
SPIN-code: 3824-2646

Doctor of Medical Sciences, Corresponding Member of RAS, Professor, Human Anatomy and Histology Department

Russian Federation, Trubetskaya str., 8/2, Moscow, 119048

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