Activation of the mapk signal cascade components phosphorylation involved in the formation of the G0-positive tumor phenotype
- 作者: Esimbekova A.R.1, Lapkina E.Z.1, Ruksha T.G.1
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隶属关系:
- Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
- 期: 卷 21, 编号 2 (2023)
- 页面: 33-38
- 栏目: Original research
- URL: https://ogarev-online.ru/1728-2918/article/view/143153
- DOI: https://doi.org/10.29296/24999490-2023-02-05
- ID: 143153
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详细
Introduction. Among the heterogeneous population of tumor cells, there are so-called dormant and senescent cells located in the G0 phase of the cell cycle. The transition to the G0 phase is a stress response mediated, for example, by treatment with chemotherapeutic drugs. The functioning of such cells is associated with the development of non-response.
The aim of the study. G0-positive skin melanoma cells modulation with subsequent assessment of the MARK signal cascade molecules, including the main tumor suppressor p53.
Material and methods. Skin melanoma cells were incubated with the cytostatic drug dacarbazine to induce the level of G0-positive cells. Total RNA extracted from cells was used for transcriptome analysis, after which the level of phosphorylation of MARK key molecules was evaluated. By immunocytochemistry (ICC) and real-time PCR (PCR-RT) the activity of tumor suppressor p53 was analyzed.
Results. As a result of the G0-positive cells level modulation, the MARK signal cascade is among the signaling pathways with the largest number of genes with altered expression. Significantly increased the number of phosphorylated proteins JNK, p70S6K, MEK, RSK1 and RSK2, as well as protein p53, capable of forming a senescent phenotype of tumor cells.
Conclusion. When the level of G0-positive skin melanoma cells is modulated by the cytostatic drug dacarbazine, phosphorylation of the MARK signaling cascade components involved in the formation of the G0-positive tumor phenotype increases.
作者简介
Aleksandra Esimbekova
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
编辑信件的主要联系方式.
Email: aleksandra.esimbekova.96@mail.ru
ORCID iD: 0000-0001-6363-5941
PhD student of the Department of Pathophysiology
俄罗斯联邦, P. Zeleznyak street, 1, Krasnoyarsk, 660022Ekaterina Lapkina
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Email: e.z.lapkina@mail.ru
ORCID iD: 0000-0002-7226-9565
Associate Professor of the Department of Pharmacy, Candidate of biological sciences
俄罗斯联邦, P. Zeleznyak street, 1, Krasnoyarsk, 660022Tatiana Ruksha
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Email: tatyana_ruksha@mail.ru
ORCID iD: 0000-0001-8142-4283
Head of the Department of Pathophysiology, Doctor of medical sciences, Professor
俄罗斯联邦, P. Zeleznyak street, 1, Krasnoyarsk, 660022参考
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