电邮这篇文章 
Genetic updating and marks of cellular lines
- 作者: Moskalev A.V.1, Gumilevskiy B.Y.1, Apchel A.V.2, Tsygan V.N.1
-
隶属关系:
- Military medical academy of S.M. Kirov
- Northwest Medical Training Center for Postgraduate Education
- 期: 卷 22, 编号 3 (2020)
- 页面: 168-175
- 栏目: Literature reviews
- URL: https://ogarev-online.ru/1682-7392/article/view/50555
- DOI: https://doi.org/10.17816/brmma50555
- ID: 50555
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
Abstract. Despite great advances in the biology of stem cells, there are still many dark spots. Genetic modification techniques, which can be used to track the lines of different cells, primarily stem cells, help to solve this problem. Various methods of biotechnology research are considered, allowing to evaluate the options of introducing new genes into cells and even whole organisms, as well as methods of controlling their expression in time and space, their activation, differentiation and decrease in functional activity, expression of several target genes. Options with multi-cystron vectors encoding several proteins are described. Options for introducing genes using plasmids, electroportation of their disadvantages and advantages are characterized. The most promising and the safest is a retroviral vector using lentivirus vectors capable of generating additional copies of itself, which is very important in the field of biotechnology security. A line of packing cells, usually 293T cells, is used to produce a viral vector. Prospects for the use of adenovirus and adenoassociated vectors are characterized. The achievement of modern biotechnology methods is the system of short palindrome repetitions located in groups, which is a unique tool for genome editing. At the heart of this system is the process of cutting out sequences of deoxyribonucleic acid, which are permanent and which are supported by cells regardless of subsequent divisions or changes in condition. The system allows geneticists and medical researchers to edit parts of the genome by removing, adding or modifying successive sites of deoxyribonucleic acid. An important problem with biotechnology methods is how to control the expression of transgenes. Today, it is quite effective to control expression with a factor present in the gene delivery vector itself and which is only active in a certain type of cell. Endonuclease bacteriophage P1 is used to regulate transgene expression, which cuts deoxyribonucleic acid only at specific sites. This system is introduced in both eukaryotic and prokaryotic systems.
作者简介
A. Moskalev
Military medical academy of S.M. Kirov
编辑信件的主要联系方式.
Email: vmeda-nio@mil.ru
俄罗斯联邦, Saint Petersburg
B. Gumilevskiy
Military medical academy of S.M. Kirov
Email: vmeda-nio@mil.ru
俄罗斯联邦, Saint Petersburg
A. Apchel
Northwest Medical Training Center for Postgraduate Education
Email: vmeda-nio@mil.ru
俄罗斯联邦, Saint Petersburg
V. Tsygan
Military medical academy of S.M. Kirov
Email: vmeda-nio@mil.ru
俄罗斯联邦, Saint Petersburg
参考
- Владимирская, Е.Б. Биологические основы и перспективы терапии стволовыми клетками / Е.Б. Владимирская, О.А. Майорова, С.А. Румянцев. – М.: Медицина и здоровье, 2007. – 392 с.
- Москалев, А.В. Общая иммунология с основами клинической иммунологии / А.В. Москалев, В.Б. Сбойчаков, А.С. Рудой. – М.: Гэотар-Медиа, 2015. – 351 с.
- Москалев, А.В. Аутоиммунные заболевания. Диагностика и лечение / А.В. Москалев [и др.]. – М.: Гэотар-Медиа, 2017. – 218 с.
- Ярилин, А. А. Иммунология / А.А. Ярилин. – М.: Гэотар-Медиа, 2010. – 957 с.
- Abbas, A.K. Cellular and Molecular Immunology. – 9-th edition / A.K. Abbas, A.H. Lichtman, S. Pillai. – Philadelphia, Pennsylvania: W. B. Saunders Company, 2018. – 565 p.
- Вhaya, D. CRISPR-Cas systems in bacteria and archaea: Versatile small RNAs for adaptive defense and regulation / D. Bbhay [et al.] // Annu. Rev. Genet. – 2011. – Vol. 45. – P. 273–297.
- Cai, L. Suppression of hepatocyte growth factor production impairs the ability of adipose-derived stem cells to promote ischemic tissue revascularization / L. Cai [et al.] // Stem Cells. – 2007. – Vol. 25. – P. 3234–3243.
- Dominici, M. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement / M. Dominici [et al.] // Cytotherapy. – 2006. – Vol. 8. – P. 315–317.
- Hilton, I.B. Epigenome editing by a CRISPR-Cas9 – based acetyltransferase activates genes from promoters and enhancers / I.B. Hilton [et al.] // Nat. Biotechnol. – 2015. – Vol. 33. – P. 510–517.
- Jeon, E.S. Sphingosylphosphorylcholine induces proliferation of human adipose tissue derived mesenchymal stem cells via activation of JNK / E.S. Jeon [et al.] // J. Lipid Res. – 2006. – Vol. 47. – P. 653–664.
- Gilbert, L.A. CRISPR–mediated modular RNA-guided regulation of transcription in eukaryotes / L.A. Gilbert [et al.] // Cell, – 2013. – Vol. 154. – P. 442–451.
- Kern, S.E. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue / S.E. Kern [et al.] // Stem Cells. – 2006. – Vol. 24. – P. 1294–1301.
- Lee, J. Human adipose-derived stem cells display myogenic potential and perturbed function in hypoxic conditions / J. Lee [et al.] // Biochem. Biophys. Res. Commun. – 2006. – Vol. 341. – P. 882–888.
- Li, B. Adipose tissue stromal cells transplantation in rats of acute myocardial infarction / B. Li [et al.] // Coron Artery Dis. – 2007. – Vol. 18. – P. 221–227.
- Lipinski, M.J. Impact o f intracoronary cell therapy on left ventricular function in the setting of acute myocardial infarction: a collaborative systematic review and meta-analysis of controlled clinical trials / M.J. Lipinski [et al.] // J. Am. Coli. Cardiol. – 2007. – Vol. 50. – P. 1761–1767.
- McDonald, J.I. Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation / J.I. McDonald [et al.] // Biol. Open. – 2016. – Vol. 5. – P. 866–874.
- Miyahara, Y. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction / Y. Miyahara [et al.] // Nat Med. – 2006. – Vol. 12, № 4. – P. 459–465.
- Olson, K. Contemporary clinical immunology and serology / K. Olson, E. De Nardin. – New Jersey: Upper Saddle River, 2013. – 439 p.
- Rose, N.R. The autoimmune diseases. – fith edition / N.R. Rose, I.R. Mackay. – Philadelphia, 2018. – 1265 p.
- Schaffler, A. Concise review: Adipose Tissue derived stromal cells – basic and clinical implications for novel cell-based therapies / A. Schaffler, C. Buchler // Stem Cells. – 2007. – Vol. 25. – P. 818–827.
- Sternberg, S.H. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9 / S.H. Sterberg // Nature. – 2014. – Vol. 507. – P. 62–67.
- Thakore, P.I. Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements / P.I. Thakore [et al.] // Nat. Methods. – 2015. – Vol. 12. – P. 1143–1149.
- Wu, Y. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis / Y. Wu [et al.] // Stem Cells. – 2007. – Vol. 25. – P. 2648–2659.
- Zabriskie, J.B. Essential clinical immunology / J.B. Zabriskie – N. Y., 2009. – 362 p.
- Zetsche, B. Cpf1 is a single RNA – guided endonuclease of a class 2 CRISPR-Cas system / B. Zetsche [et al.] // Cell. – 2016. – Vol. 163. – P. 759–771.
补充文件
