Development of a technique for quantitative determination of silver by extraction-photometric method in a polyacrylamide-based medical device
- Authors: Paskar I.V.1, Senchenko S.P.1, Voroncova I.S.1, Paskar N.G.2, Mironova М.G.3
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Affiliations:
- LLC Research and testing center «FARMOBORONA»
- I.M. Sechenov First Moscow State Medical University (Sechenov University) Ministry of Health of the Russian Federation
- LLC Scientific Center BIOFORM
- Issue: Vol 28, No 4 (2025)
- Pages: 32-40
- Section: Pharmaceutical chemistry
- URL: https://ogarev-online.ru/1560-9596/article/view/290926
- ID: 290926
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Abstract
Introduction. One of the promising directions of using polymer hydrogels in medicine is the production of synovial fluid endoprostheses. Thus, cross-linked polyacrylamide serves as the basis for the medical device “Sterile Silver Ion Waterborne Biopolymer Material”, which is used for intra-articular injections in the treatment of osteoarthritis. The addition of silver ions to polymeric hydrogels is due to its broad spectrum of antimicrobial activity and is used to prevent microbial contamination. However, in order to safely use a medical device containing silver ions and avoid the risk of heavy metal poisoning, the content of this component must be monitored. Among the various methods of quantitative analysis of metals, the extraction-photometric method is the easiest to reproduce and does not require expensive equipment, which allows to control the content of the element in low content.
Purpose of the study. Development of a technique for quantitative determination of silver in silver-containing medical device based on polyacrylamide hydrogel by extraction-photometric method.
Material and Methods. A sample of medical product “Material-biopolymer water-containing with silver ions sterile”, produced by LLC “NC ‘BIOFORM’, Russia, was used as an object of the study. The silver content in the studied object was determined by the extraction-photometric method.
Results. The technique of quantitative determination of silver in the analyzed medical device by the extraction-photometric method based on the extraction of pre-ionized silver in an organic solvent (dithizone solution in 1-butanol 0.0025 %) through the formation of a high-affinity complex in acidic medium was developed. The absence of placebo (polyacrylamide) influence on determination of silver ions was proved.
Conclusions. This study demonstrated the possibility of silver ions determination in the medical device “Material-biopolymer water-containing with silver ions sterile” after extraction with dithizone solution in 1-butanol 0.0025 % by extraction-photometric method.
It was found that the developed method is applicable in the range of measured concentrations of silver ions from 0.2 to 5.0 μg/mL, the average value of the method opening on 6 levels of silver content is 101.5 %.
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##article.viewOnOriginalSite##About the authors
I. V. Paskar
LLC Research and testing center «FARMOBORONA»
Author for correspondence.
Email: paskar_irina@farmoborona.ru
Ph.D. (Pharm.), Managing Director
Russian Federation, 46 A, Gagarina str., Korolev, Moscow region, 141074S. P. Senchenko
LLC Research and testing center «FARMOBORONA»
Email: senchenko_sergey@farmoborona.ru
ORCID iD: 0000-0003-0212-3840
SPIN-code: 4860-0144
Dr.Sc. (Pharm.), Associate Professor, Head of Department of Analytical Methods Development
Russian Federation, 46 A, Gagarina str., Korolev, Moscow region, 141074I. S. Voroncova
LLC Research and testing center «FARMOBORONA»
Email: voroncova_irina@farmoborona.ru
Pharmacist of Department of Analytical Methods Development
Russian Federation, 46 A, Gagarina str., Korolev, Moscow region, 141074N. G. Paskar
I.M. Sechenov First Moscow State Medical University (Sechenov University) Ministry of Health of the Russian Federation
Email: nnikpaskar@yandex.ru
Student of the Department of Pharmacy, A.P. Nelyubin Institute of Pharmacy
Russian Federation, 8/2, Trubetskaya str., Mosсow, 119991М. G. Mironova
LLC Scientific Center BIOFORM
Email: paskar_irina@farmoborona.ru
Head of Analytical Laboratory
Russian Federation, Kominterna str. 7 k. 2, premises. 1/3, Moscow, 129327References
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