Phenolic precursors as regulators of the polyphenols accumulation in the tea plant callus cultures
- Authors: Aksenova M.A.1, Nechaeva T.L.1, Zagoskina N.V.1
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Affiliations:
- K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences
- Issue: Vol 28, No 12 (2025)
- Pages: 135-141
- Section: Problems of experimental biology and medicine
- URL: https://ogarev-online.ru/1560-9596/article/view/362645
- DOI: https://doi.org/10.29296/25877313-2025-12-16
- ID: 362645
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Abstract
Introduction. Plant tissue cultures are potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. In most cases, their accumulation is lower than in the initial explants, which requires a search for factors and influences to intensify this process. In this case, it is very promising to use the precursors of their biosynthesis as potential “regulators" of the formation of various metabolites.
The aim of the study was to study the effect of L-phenylalanine (FA, 3 mM), trans-cinnamic acid (CC, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on the accumulation of various classes of phenolic compounds, including phenylpropanoids and flavanols, in callus cultures of the tea plant (Camellia sinensis L.): a strain of "Kimyn" and "Local population".
Material and methods: The object of the study was callus cultures of tea plants of leaf origin grown for 7 days on a liquid nutrient medium of Heller basic composition (control) or enriched with L-phenylalanine (3 mM), trans-cinnamic acid (1 mM) or naringenin (0.5 mM). The morphophysiological parameters of the cullus (color, density, water content), the amount of polyphenols and flavans in them were analyzed.
Results. According to the data obtained, the action of NG led to a significant increase in the accumulation of phenylpropanoids and flavanols in two strains of tea culture. Whereas FA contributed to a sharp increase in the content of phenylpropanoids only, which is more pronounced in the "Kimyn" strain. Thus, the most effective "regulators" of the accumulation of phenolic compounds are FA and NG. At the same time, the trends in the accumulation of various polyphenols were similar for both cultures, however, in the case of the "Wormwood" strain, the response was more pronounced.
Conclusion. The study demonstrates the significant biotechnological potential of phenolic precursors as regulators of the accumulation of polyphenols in the callus cultures of the tea plant. The effect of their action depends on both the compound used and the strain of the tea plant. The results obtained open up prospects for the development of effective biotechnological approaches to the regulation of the accumulation of pharmacologically valuable secondary metabolites of a phenolic nature in in vitro cultures of higher plants.
About the authors
M. A. Aksenova
K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences
Author for correspondence.
Email: aksenova@ifr.moscow
ORCID iD: 0000-0002-3228-3311
SPIN-code: 5281-5794
Research Scientist
Russian Federation, 35 Botanicheskay str., Moscow, 127276T. L. Nechaeva
K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences
Email: nechaeva@ifr.moscow
ORCID iD: 0000-0003-3341-4763
SPIN-code: 7700-6475
Research Scientist
Russian Federation, 35 Botanicheskay str., Moscow, 127276N. V. Zagoskina
K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences
Email: zagoskina@ifr.moscow
ORCID iD: 0000-0002-1457-9450
SPIN-code: 1948-0478
Dr.Sc. (Biol.), Professor, Leading Research Scientist
Russian Federation, 35 Botanicheskay str., Moscow, 127276References
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