Effect of Akkermansia muciniphila-based probiotic on small intestinal morphology and gene expression and behavioral characteristics of mice under induced systemic inflammation
- Authors: Pogorelova S.V.1, Chirkin E.A.1, Shutikov V.A.2, Mikhailov E.V.2, Syromyatnikov M.Y.1
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Affiliations:
- Voronezh State University of Engineering Technologies
- All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
- Issue: Vol 28, No 12 (2025)
- Pages: 126-134
- Section: Problems of experimental biology and medicine
- URL: https://ogarev-online.ru/1560-9596/article/view/362644
- DOI: https://doi.org/10.29296/25877313-2025-12-15
- ID: 362644
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Abstract
Introduction. The microbiome plays an important role in the normal functioning of the body, an imbalance in its composition leads to various diseases. Currently, many studies are being conducted on the bacterium Akkermansia muciniphila, which is a new generation probiotic that has a beneficial effect on the composition of the microbiome. However, the mechanism of the effect of the bacterium on inflammatory processes occurring in the body and induced by various drugs has not been fully studied.
The aim of the study was to determine the effect of A. muciniphila on serum biochemical parameters, as well as behavioral and physiological characteristics of mice during lipopolysaccharide (LPS)-induced systemic inflammation.
Material and methods. The following methods were used in the study: physiological test "Open field", biochemical analysis of blood serum using the automatic analyzer "VitaLine 200", histological examination of the small intestine was carried out using hematoxylin and eosin staining, gene expression was assessed by RT-PCR combined with reverse transcription. Statistical processing was performed in the R environment (Version 4.1.1) and Statistica 12, using the Spearman correlation coefficient, the Kruskal–Wallis test and the Bonferroni correction, one-way variance test (ANOVA) using the Tukey post-hoc test.
Results. A. muciniphila inhibited the inflammatory process induced by LPS injections and, as a result, led to an increase in research activity. The restoration of the small intestine structure was observed. Also, against the background of the probiotic effect, normalization of the biochemical parameters of the blood serum was revealed.
Conclusion. With the combined administration of LPS and A. muciniphila, antioxidant protection (GCLC) and intestinal barrier function (ZO-1) were enhanced, thus, the probiotic demonstrates therapeutic potential in the course of the inflammatory process.
About the authors
S. V. Pogorelova
Voronezh State University of Engineering Technologies
Author for correspondence.
Email: zubkowa.sweta@gmail.com
ORCID iD: 0009-0002-7924-8027
SPIN-code: 4269-6171
Junior Research Scientist, Laboratory of Metagenomics and Food Biotechnology
Russian Federation, 19 Revolution Avenue, Voronezh, 394036E. A. Chirkin
Voronezh State University of Engineering Technologies
Email: chirkin@bio.vsu.ru
ORCID iD: 0009-0000-0021-6408
SPIN-code: 2757-7601
Student, Technician, Laboratory of Metagenomics and Food Biotechnology
Russian Federation, 19 Revolution Avenue, Voronezh, 394036V. A. Shutikov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Email: shutikov.02@yandex.ru
ORCID iD: 0009-0004-2018-2662
SPIN-code: 1167-3528
Junior Research Scientist, Laboratory of Innovative Recombinant Proteomics Preparations
Russian Federation, 114b Lomonosov str., Voronezh, 394087E. V. Mikhailov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Email: voronezh81@rambler.ru
ORCID iD: 0000-0001-5457-1325
SPIN-code: 9506-5052
Ph.D. (Vet.), Head of the Department of Experimental Pharmacology and Functioning of Living Systems
Russian Federation, 114b Lomonosov str., Voronezh, 394087M. Yu. Syromyatnikov
Voronezh State University of Engineering Technologies
Email: syromyatnikov@bio.vsu.ru
ORCID iD: 0000-0001-9028-0613
SPIN-code: 7321-3642
Ph.D. (Biol.), Leading Research Scientist, Laboratory of Metagenomics and Food Biotechnology, Associate Professor, Department of Genetics, Cytology and Bioengineering
Russian Federation, 19 Revolution Avenue, Voronezh, 394036References
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